caa a22 4500 477083803 CHVBK 20180405111457.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF01919513 doi (NATIONALLICENCE)springer-10.1007/BF01919513 Quantification of Mycobacterium tuberculosis DNA using PCR and a simple dot blot-based DNA enzyme assay (DEA) [Elektronische Daten] [M. Kaffarnik, H. Mauch, A. Roth] Conclusions: Use of PCR for quantification of microorganisms has certain limitations. Methods currently under investigation, such as covalent binding of modified DNA onto the surface of microwells or coupling to magnetic beads with the aid of biotin-avidin, are hampered by problems concerning immobilization of DNA on a solid phase. Moreover, these strategies are laborious, including several washing steps and complicated detection systems (sandwich hybridization). We have developed an alternative method, exploiting the reliability of covalent DNA binding to positively charged nylon membranes enabling easy to handle direct hybridization. The method is adaptable for routine use in clinical laboratories. We have shown results of a quantitative assay to measure bacterial load of MTB. Quantification of MTB may have value in: (i) monitoring patients under anti-myobacterial therapy, and (ii) early in vitro drug susceptibility testing. Birkhäuser Verlag, 1996 Kaffarnik M. Institut für Mikrobiologie und Immunologie, Krankenhaus Zehlendorf-Heckeshorn, Zum Heckeshorn 33, D-14109, Berlin, Germany aut Mauch H. Institut für Mikrobiologie und Immunologie, Krankenhaus Zehlendorf-Heckeshorn, Zum Heckeshorn 33, D-14109, Berlin, Germany aut Roth A. Institut für Mikrobiologie und Immunologie, Krankenhaus Zehlendorf-Heckeshorn, Zum Heckeshorn 33, D-14109, Berlin, Germany aut Experientia Birkhäuser-Verlag 52/4(1996-04-01), 298-298 0014-4754 52:4<298 1996 52 18 https://doi.org/10.1007/BF01919513 text/html Onlinezugriff via DOI 1 abstract jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01919513 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kaffarnik M. Institut für Mikrobiologie und Immunologie, Krankenhaus Zehlendorf-Heckeshorn, Zum Heckeshorn 33, D-14109, Berlin, Germany aut NATIONALLICENCE 700 1- Mauch H. Institut für Mikrobiologie und Immunologie, Krankenhaus Zehlendorf-Heckeshorn, Zum Heckeshorn 33, D-14109, Berlin, Germany aut NATIONALLICENCE 700 1- Roth A. Institut für Mikrobiologie und Immunologie, Krankenhaus Zehlendorf-Heckeshorn, Zum Heckeshorn 33, D-14109, Berlin, Germany aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/4(1996-04-01), 298-298 0014-4754 52:4<298 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer