caa a22 4500 477083889 CHVBK 20180405111457.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF01919538 doi (NATIONALLICENCE)springer-10.1007/BF01919538 N-Monomethyl-arginine and nicotinamide prevent streptozotocin-induced double strand DNA break formation in pancreatic rat islets [Elektronische Daten] [F. Bedoya, F. Solano, M. Lucas] The impact of short term in vitro exposure to the diabetogenic drug streptozotocin on pancreatic islet glucose metabolism, insulin secretion, DNA fragmentation and cell viability, was studied. Streptozotocin impaired cell viability as well as insulin secretion and the oxidation of glucose. These effects were partially counteracted by inhibition of the inducible form of nitric oxide synthase with N-monomethyl-arginine and by scavenging oxygen free radicals with nicotinamide. Isolated islets underwent double strand DNA fragmentation after 24 h in culture. The degree of DNA breakdown was strongly enhanced by exposure of the islet DNA was obtained with N-monomethyl-arginine and nicotinamide. These data suggest that the generation of reactive oxygen and nitrogen species is involved in the deleterious action of streptozotocin on pancreatic islet tissue. A role for oxygen radicals generated during streptozotocin-induced islet cell damage as possible mediators of the expression of the inducible form of nitric oxide synthase and the scavenging action of nicotinamide on these radicals, is then proposed. Birkhäuser Verlag, 1996 Pancreatic islets nationallicence DNA degradation nationallicence streptozotocin nationallicence nicotinamide nationallicence nitric oxide nationallicence Bedoya F. Laboratorio de Bioquímica de Sistemas Immunólogicos, Departamento de Bioquímica Médica y Biología Molecular, Facultad de Medicina, Universidad de Sevilla, Avad. Sánchez Pizjuán 4, E-41009, Sevilla, (Spain) aut Solano F. Laboratorio de Genetica Molecular, Departamento de Bioquímica Médica y Biologia Molecular, Facultad de Medicina, Universidad de Sevilla, Avda. Sanchez Pizjuán 4, E-41009, Sevilla, (Spain) aut Lucas M. Laboratorio de Genetica Molecular, Departamento de Bioquímica Médica y Biologia Molecular, Facultad de Medicina, Universidad de Sevilla, Avda. Sanchez Pizjuán 4, E-41009, Sevilla, (Spain) aut Experientia Birkhäuser-Verlag 52/4(1996-04-01), 344-347 0014-4754 52:4<344 1996 52 18 https://doi.org/10.1007/BF01919538 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01919538 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bedoya F. Laboratorio de Bioquímica de Sistemas Immunólogicos, Departamento de Bioquímica Médica y Biología Molecular, Facultad de Medicina, Universidad de Sevilla, Avad. Sánchez Pizjuán 4, E-41009, Sevilla, (Spain) aut NATIONALLICENCE 700 1- Solano F. Laboratorio de Genetica Molecular, Departamento de Bioquímica Médica y Biologia Molecular, Facultad de Medicina, Universidad de Sevilla, Avda. Sanchez Pizjuán 4, E-41009, Sevilla, (Spain) aut NATIONALLICENCE 700 1- Lucas M. Laboratorio de Genetica Molecular, Departamento de Bioquímica Médica y Biologia Molecular, Facultad de Medicina, Universidad de Sevilla, Avda. Sanchez Pizjuán 4, E-41009, Sevilla, (Spain) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/4(1996-04-01), 344-347 0014-4754 52:4<344 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer