caa a22 4500 477083935 CHVBK 20180405111457.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF01919508 doi (NATIONALLICENCE)springer-10.1007/BF01919508 Effect of genome size and rrn gene copy numbers of PCR amplification products of 16S rRNA genes from mixed bacterial species [Elektronische Daten] [V. Farrelly, F. Rainey, E. Stackebrandt] Results and conclusion: As determined by image analysis of SYBR green-stained amplification products the experimentally determined ratio corresponded well with the expected ratio calculated from the number ofrrn genes per equimolar amount of DNA in mixtures containing DNA ofEscherichia coli and ‘Thermus thermophilus' and DNA ofPseudomonas aeruginosa and ‘T. thermophilus'. The values for the pairBacillus subtilis and ‘T. thermophilus' showed higher deviation from the predicted value. The dependence of the amount of 16S rDNA amplification products on these two parameters makes it impossible to quantify the number of species present in 16S rDNA clone library of an environmental sample, as long as these two parameters are unknown for these species. Birkhäuser Verlag, 1996 Farrelly V. DSM-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124, Braunschweig, Germany aut Rainey F. DSM-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124, Braunschweig, Germany aut Stackebrandt E. DSM-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124, Braunschweig, Germany aut Experientia Birkhäuser-Verlag 52/4(1996-04-01), 294-295 0014-4754 52:4<294 1996 52 18 https://doi.org/10.1007/BF01919508 text/html Onlinezugriff via DOI 1 abstract jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01919508 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Farrelly V. DSM-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124, Braunschweig, Germany aut NATIONALLICENCE 700 1- Rainey F. DSM-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124, Braunschweig, Germany aut NATIONALLICENCE 700 1- Stackebrandt E. DSM-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124, Braunschweig, Germany aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/4(1996-04-01), 294-295 0014-4754 52:4<294 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer