caa a22 4500 477083986 CHVBK 20180405111457.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF01923984 doi (NATIONALLICENCE)springer-10.1007/BF01923984 Synthesis of a ubiquitously present new HSP60 family protein is enhanced by heat shock only in the Malpighian tubules of Drosophila [Elektronische Daten] [S. Lakhotia, B. Singh] A homologue of the chaperonin protein of the HSP60 family has not been shown so far inDrosophila. Using an antibody specific to HSP60 family protein in Western blotting and immunocytochemistry, we showed that a 64-kDa polypeptide, homologous to the HSP60, is constitutively present in all tissues ofDrosophila melanogaster throughout the life cycle from the freshly laid egg to all embryonic, larval and adult stages. A 64-kDa polypeptide reacting with the same antibody in Western blots is present in all species ofDrosophila examined. Using Western blotting in conjunction with35S-methionine labeling of newly synthesized proteins and immuno-precipitation of the labeled proteins with HSP60-specific antibody, it was shown that synthesis of the 64-kDa homologue of HSP60 is appreciably increased by heat shock only in the Malpighian tubules, which are already known to lack the common HSPs. Birkhäuser Verlag Basel, 1996 Chaperonin nationallicence Drosophila nationallicence groEL nationallicence heat shock nationallicence heat shock proteins nationallicence HSP60 nationallicence Malpighian tubules nationallicence TCP-1 nationallicence Lakhotia S. Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, 221 005, Varanasi, (India) aut Singh B. Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, 221 005, Varanasi, (India) aut Experientia Birkhäuser-Verlag 52/8(1996-08-01), 751-756 0014-4754 52:8<751 1996 52 18 https://doi.org/10.1007/BF01923984 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01923984 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lakhotia S. Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, 221 005, Varanasi, (India) aut NATIONALLICENCE 700 1- Singh B. Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, 221 005, Varanasi, (India) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/8(1996-08-01), 751-756 0014-4754 52:8<751 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer