caa a22 4500 477084001 CHVBK 20180405111457.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF01923992 doi (NATIONALLICENCE)springer-10.1007/BF01923992 Isolation of type II epithelial cells from rabbit fetal lungs by adherence on an IgG-coated surface [Elektronische Daten] [A. Gupta, S. Kaushal, S. Majumdar, S. Sanyal] The lung is comprised of about 40 different cell types, of which only 15% are type II cells. These are the major, if not the sole, source of synthesis and secretion of lung surfactant. To date a large number of methods have been described for the isolation of pure populations of type II cells using a wide variety of techniques, but most of these have employed differential centrifugation methods and have used adult rodents. The present study reports the isolation of type II cells from fetal rabbit lungs by the immunoglobin G plating method. Pure populations of fetal type II cells in high yield and with good viability were obtained by the procedure for the first time from rabbit fetal tissue. Birkhäuser Verlag Basel, 1996 Pulmonary surfactant nationallicence lung type II epithelial cell isolation nationallicence adherence on IgG plates nationallicence Gupta A. Department of Experimental, Medicine, Postgraduate Institute of Medical Education and Research, 160012, Chandigarh, (India) aut Kaushal S. Department of Experimental, Medicine, Postgraduate Institute of Medical Education and Research, 160012, Chandigarh, (India) aut Majumdar S. Department of Experimental, Medicine, Postgraduate Institute of Medical Education and Research, 160012, Chandigarh, (India) aut Sanyal S. Department of Biophysics, Panjab University, 160014, Chandigarh, (India) aut Experientia Birkhäuser-Verlag 52/8(1996-08-01), 799-802 0014-4754 52:8<799 1996 52 18 https://doi.org/10.1007/BF01923992 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01923992 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gupta A. Department of Experimental, Medicine, Postgraduate Institute of Medical Education and Research, 160012, Chandigarh, (India) aut NATIONALLICENCE 700 1- Kaushal S. Department of Experimental, Medicine, Postgraduate Institute of Medical Education and Research, 160012, Chandigarh, (India) aut NATIONALLICENCE 700 1- Majumdar S. Department of Experimental, Medicine, Postgraduate Institute of Medical Education and Research, 160012, Chandigarh, (India) aut NATIONALLICENCE 700 1- Sanyal S. Department of Biophysics, Panjab University, 160014, Chandigarh, (India) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/8(1996-08-01), 799-802 0014-4754 52:8<799 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer