caa a22 4500 47708401X CHVBK 20180405111457.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF01923995 doi (NATIONALLICENCE)springer-10.1007/BF01923995 Inhibition mechanisms of HIV-1, Mo-MuLV and AMV reverse transcriptases by Kelletinin A from Buccinulum corneum [Elektronische Daten] [P. Orlando, G. Strazzullo, F. Carretta, M. De Falco, P. Grippo] Kelletinin A [ribityl pentakis (p-hydroxybenzoate)] (KA), an inhibitor of HTLV-1 replication isolated fromBuccinulum corneum, showed a noncompetitive inhibitory activity with respect to the template primer and to dTTP in the poly(rA) oligo(dT)12-18-directed reaction of HIV-1, Mo-MuLV and AMV reverse trancriptases (RT). Analysis of natural and synthetic KA-related compounds showed that the inhibitory activity was strictly related to the structural peculiarities of the molecule. In the presence of DNA as template primer the inhibition mechanism was drastically modified: HIV-1 RT activity was stimulated by low concentrations of KA and was inhibited by increasing the concentration of the compound, while Mo-MuLV and AMV activities were irreversibly inhibited by the formation of a non-reactive complex. The RNase H activities of these RTs were not affected by KA. The results of this study suggest a different mechanism of interaction of Kelletinins with HIV-1 RT compared with other non-nucleoside inhibitors. A possible use of these drugs in combination therapy and in the design of structurebased reverse transcriptase inhibitors is discussed. Birkhäuser Verlag Basel, 1996 Kelletinin A nationallicence human immunodeficiency virus type 1 (HIV-1) nationallicence Moloney murine leukemia virus (Mo-MuLV) nationallicence avian myeloblastosis virus (AMV) nationallicence reverse-transcriptases nationallicence inhibition nationallicence Orlando P. Istituto Biochimica delle Proteine ed Enzimologia, CNR, Via Toiano 6, I-80072, Arco Felice, Naples, (Italy) aut Strazzullo G. Istituto Chimica Molecole Interesse Biologico of CNR, Via Toiano 6, I-80072, Arco Felice, Naples, (Italy) aut Carretta F. Istituto Biochimica delle Proteine ed Enzimologia, CNR, Via Toiano 6, I-80072, Arco Felice, Naples, (Italy) aut De Falco M. Istituto Biochimica delle Proteine ed Enzimologia, CNR, Via Toiano 6, I-80072, Arco Felice, Naples, (Italy) aut Grippo P. Istituto Biochimica Macromolecole, Faculty of Medicine, II University of Naples, Via Costantinopoli 16, I-80138, Naples, (Italy) aut Experientia Birkhäuser-Verlag 52/8(1996-08-01), 812-817 0014-4754 52:8<812 1996 52 18 https://doi.org/10.1007/BF01923995 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01923995 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Orlando P. Istituto Biochimica delle Proteine ed Enzimologia, CNR, Via Toiano 6, I-80072, Arco Felice, Naples, (Italy) aut NATIONALLICENCE 700 1- Strazzullo G. Istituto Chimica Molecole Interesse Biologico of CNR, Via Toiano 6, I-80072, Arco Felice, Naples, (Italy) aut NATIONALLICENCE 700 1- Carretta F. Istituto Biochimica delle Proteine ed Enzimologia, CNR, Via Toiano 6, I-80072, Arco Felice, Naples, (Italy) aut NATIONALLICENCE 700 1- De Falco M. Istituto Biochimica delle Proteine ed Enzimologia, CNR, Via Toiano 6, I-80072, Arco Felice, Naples, (Italy) aut NATIONALLICENCE 700 1- Grippo P. Istituto Biochimica Macromolecole, Faculty of Medicine, II University of Naples, Via Costantinopoli 16, I-80138, Naples, (Italy) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/8(1996-08-01), 812-817 0014-4754 52:8<812 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer