caa a22 4500 477084052 CHVBK 20180405111457.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF01923993 doi (NATIONALLICENCE)springer-10.1007/BF01923993 Immunochemical characterization of antibody-coated nanoparticles [Elektronische Daten] [F. Velge-Roussel, P. Breton, X. Guillon, F. Lescure, N. Bru, D. Bout, J. Hoebeke] A new method using surface plasmon resonance (SPR) through the BIAcore was used to demonstrate the specific interaction between an anti-CD4 monoclonal antibody (IOT4a), adsorbed on poly(methylidene malonate 2.1.2) (PMM 2.1.2) nanoparticles, and the CD4 molecule. The results obtained were compared with the interaction of the same immunonanoparticles with rabbit anti-mouse Fc antibodies. The molar ratio (Fc)/(Fab) was 1, suggesting that the same number of epitopes on the Fc and the Fab fragments were accessible after IOT4a adsorption onto nanoparticles. Comparing the observed association rates of free antibody and antibody adsorbed on nanoparticles, the number of molecules of IOT4a antibody on PMM 2.1.2 nanoparticles was estimated as between 2.6 and 3 per nanoparticle. The properties of the antibody-coated nanoparticles are compatible with their use as antibody-targeted pharmacophores. Birkhäuser Verlag Basel, 1996 Immunonanoparticle nationallicence methylidene malonate nationallicence surface plasmon resonance nationallicence rsCD4 : recombinant soluble cluster differentiation 4 nationallicence HIV-1 : human immunodeficiency virus type 1 nationallicence RU : resonance unit nationallicence Np : nanoparticles nationallicence Np-Ab : anti-CD4 immunonanoparticles nationallicence Velge-Roussel F. Immunologie des Maladies Infectieuses, Equipe associée INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques, CJF INSERM 93-09, 31 avenue Monge, F-37200, Tours, (France) aut Breton P. Laboratoires UPSA, 128 rue Danton, BP 325, F-92506, Rueil-Malmaison Cédex, (France) aut Guillon X. Laboratoires UPSA, 128 rue Danton, BP 325, F-92506, Rueil-Malmaison Cédex, (France) aut Lescure F. Laboratoires UPSA, 128 rue Danton, BP 325, F-92506, Rueil-Malmaison Cédex, (France) aut Bru N. VIRSOL, 46 rue Boissière, F-75116, Paris, (France) aut Bout D. Immunologie des Maladies Infectieuses, Equipe associée INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques, CJF INSERM 93-09, 31 avenue Monge, F-37200, Tours, (France) aut Hoebeke J. Immunologie des Maladies Infectieuses, Equipe associée INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques, CJF INSERM 93-09, 31 avenue Monge, F-37200, Tours, (France) aut Experientia Birkhäuser-Verlag 52/8(1996-08-01), 803-806 0014-4754 52:8<803 1996 52 18 https://doi.org/10.1007/BF01923993 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01923993 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Velge-Roussel F. Immunologie des Maladies Infectieuses, Equipe associée INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques, CJF INSERM 93-09, 31 avenue Monge, F-37200, Tours, (France) aut NATIONALLICENCE 700 1- Breton P. Laboratoires UPSA, 128 rue Danton, BP 325, F-92506, Rueil-Malmaison Cédex, (France) aut NATIONALLICENCE 700 1- Guillon X. Laboratoires UPSA, 128 rue Danton, BP 325, F-92506, Rueil-Malmaison Cédex, (France) aut NATIONALLICENCE 700 1- Lescure F. Laboratoires UPSA, 128 rue Danton, BP 325, F-92506, Rueil-Malmaison Cédex, (France) aut NATIONALLICENCE 700 1- Bru N. VIRSOL, 46 rue Boissière, F-75116, Paris, (France) aut NATIONALLICENCE 700 1- Bout D. Immunologie des Maladies Infectieuses, Equipe associée INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques, CJF INSERM 93-09, 31 avenue Monge, F-37200, Tours, (France) aut NATIONALLICENCE 700 1- Hoebeke J. Immunologie des Maladies Infectieuses, Equipe associée INRA d'Immunologie Parasitaire, UFR des Sciences Pharmaceutiques, CJF INSERM 93-09, 31 avenue Monge, F-37200, Tours, (France) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/8(1996-08-01), 803-806 0014-4754 52:8<803 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer