caa a22 4500 477084060 CHVBK 20180405111457.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF01923996 doi (NATIONALLICENCE)springer-10.1007/BF01923996 Characterization and relative abundance of maxi-chloride channels in Epstein-Barr virus (EBV) producer: B95-8 cells [Elektronische Daten] [T. Yeh, M. Tsai, S. Lee, M. Hsu] Several Epstein-Barr virus (EBV)-transformed cell lines were used to investigate the pathogenesis of lymphoproliferative diseases and nasopharyngeal carcinoma. The studies focus on the events occurring inside the membrane. On only one occasion, the cell membrane of EBV-transformed B lymphocytes from a cystic fibrosis patient was found to express defective Cl channels (CFTR; Cystic Fibrosis Transmembrane conductance Regulator), as in the airway epithelial cell. No other type of channel in EBV-transformed cells has so far been investigated. In this study, the cell membrane of the B95-8 cell was examined by the patch-clamp technique and compared to the non-EBV-infected BJAB cell. The high conductance (≈300 pS) maxi-chloride (Cl) channel activity was the most frequently observed event in inside-out configurations. Under similar experimental conditions, we have found a significantly higher probability of detecting maxi-Cl channel activity on the cell membrane of B95-8 cells (69%) than on BJAB cells (27%), or as previously reported on resting murine B lymphocytes (38%) or intact human T lymphocytes (37%). The relative abundance of the maxi-Cl channel on B95-8 cells may be linked to EBV infection and/or secretory ability. Birkhäuser Verlag Basel, 1996 Epstein-Barr virus nationallicence anion channel nationallicence patch clamp nationallicence Yeh T. Department of Otolaryngology, College of Medicine, National Taiwan University, Taipei, (Taiwan) aut Tsai M. Department of Pharmacology, College of Medicine, National Taiwan University, Taipei, (Taiwan) aut Lee S. Department of Otolaryngology, College of Medicine, National Taiwan University, Taipei, (Taiwan) aut Hsu M. Department of Otolaryngology, College of Medicine, National Taiwan University, Taipei, (Taiwan) aut Experientia Birkhäuser-Verlag 52/8(1996-08-01), 818-826 0014-4754 52:8<818 1996 52 18 https://doi.org/10.1007/BF01923996 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01923996 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yeh T. Department of Otolaryngology, College of Medicine, National Taiwan University, Taipei, (Taiwan) aut NATIONALLICENCE 700 1- Tsai M. Department of Pharmacology, College of Medicine, National Taiwan University, Taipei, (Taiwan) aut NATIONALLICENCE 700 1- Lee S. Department of Otolaryngology, College of Medicine, National Taiwan University, Taipei, (Taiwan) aut NATIONALLICENCE 700 1- Hsu M. Department of Otolaryngology, College of Medicine, National Taiwan University, Taipei, (Taiwan) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/8(1996-08-01), 818-826 0014-4754 52:8<818 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer