caa a22 4500 477084087 CHVBK 20180405111457.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF01923986 doi (NATIONALLICENCE)springer-10.1007/BF01923986 Phosphorylation of cockroach antennal polypeptides: effects of second messengers and pheromonal blend [Elektronische Daten] [M. Renucci, A. Tirard, L. Sreng, J. Khlat, J. Clément] In insect antennal extracts, Schleicher et al.1 showed that protein kinase C (PKC) inhibitors abolish the transience of pheromone-induced rapid inositol trisphosphate responses, which suggests that pheromonal signals act on phosphorylation of specific proteins. To confirm this hypothesis, we studied the effects of second messengers and a pheromonal blend on phosphorylation of antennal proteins in the cockroachPeriplaneta americana. Proteins from adult male antennae were phosphorylated in vitro in the presence of [γ32P] triphosphate, then separated by SDS-polyacrylamide gel electrophoresis. Numerous phosphopolypeptides were visualized. The presence of Ca++/calmodulin in the incubation medium resulted in increased phosphorylation of polypeptides with molecular weights of 38, 48, 51, 54 and 58 kDa. Stimulation of PKC by addition of Ca++ phosphatidylserine (PS)/phorbol myristate acetate (PMA) resulted in the appearance of three phosphopolypeptides of 36, 70 and 120 kDa. In the presence of cyclic adenosine monophosphate, two new major polypeptides of 46 and 42 kDa appeared; the latter polypeptide also appeared in the presence of cyclic guanosine monophosphate. Comparison with polypeptide composition of tissue from the cerci, leg, brain and fat body showed that the 36 and 48 kDa polypeptides were specific to antennae, whereas the 120 kDa polypeptide was also present in the adult brain. When antennae are subjected to pheromonal stimulation for 16 seconds prior to homogenization, in vitro phosphorylation of the 120, 70, 64 and 38 kDa polypeptides was inhibited, whereas phosphorylation of the 58, 54, 51 and 48 kDa polypeptides was strongly stimulated. It is noteworthy that a 107 kDa polypeptide was observed only after pheromonal stimulation by Ca++/PS/PMA. Our findings suggest that Ca++-and PKC-dependent protein phosphorylation systems play an important role in the transduction of pheromonal signals in antennae of male cockroachP. americana. We speculate that specific phosphoproteins may modulate sensitivity and signal amplification during the olfactory transduction process. Birkhäuser Verlag Basel, 1996 Protein phosphorylation nationallicence protein kinases nationallicence pheromones nationallicence second messengers nationallicence antennae nationallicence insect nationallicence cockroach nationallicence Renucci M. Laboratoire de Neurobiologie, UPR 9024, CNRS, 31 chemin Joseph-Aiguier, F-13402, Marseille Cedex 20, (France) aut Tirard A. Laboratoire de Neurobiologie, UPR 9024, CNRS, 31 chemin Joseph-Aiguier, F-13402, Marseille Cedex 20, (France) aut Sreng L. Laboratoire de Neurobiologie, UPR 9024, CNRS, 31 chemin Joseph-Aiguier, F-13402, Marseille Cedex 20, (France) aut Khlat J. Laboratoire de Neurobiologie, UPR 9024, CNRS, 31 chemin Joseph-Aiguier, F-13402, Marseille Cedex 20, (France) aut Clément J. Laboratoire de Neurobiologie, UPR 9024, CNRS, 31 chemin Joseph-Aiguier, F-13402, Marseille Cedex 20, (France) aut Experientia Birkhäuser-Verlag 52/8(1996-08-01), 762-768 0014-4754 52:8<762 1996 52 18 https://doi.org/10.1007/BF01923986 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01923986 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Renucci M. Laboratoire de Neurobiologie, UPR 9024, CNRS, 31 chemin Joseph-Aiguier, F-13402, Marseille Cedex 20, (France) aut NATIONALLICENCE 700 1- Tirard A. Laboratoire de Neurobiologie, UPR 9024, CNRS, 31 chemin Joseph-Aiguier, F-13402, Marseille Cedex 20, (France) aut NATIONALLICENCE 700 1- Sreng L. Laboratoire de Neurobiologie, UPR 9024, CNRS, 31 chemin Joseph-Aiguier, F-13402, Marseille Cedex 20, (France) aut NATIONALLICENCE 700 1- Khlat J. Laboratoire de Neurobiologie, UPR 9024, CNRS, 31 chemin Joseph-Aiguier, F-13402, Marseille Cedex 20, (France) aut NATIONALLICENCE 700 1- Clément J. Laboratoire de Neurobiologie, UPR 9024, CNRS, 31 chemin Joseph-Aiguier, F-13402, Marseille Cedex 20, (France) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/8(1996-08-01), 762-768 0014-4754 52:8<762 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer