caa a22 4500 477084095 CHVBK 20180405111458.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF01923985 doi (NATIONALLICENCE)springer-10.1007/BF01923985 Embryonic chick muscle produces an FGF-like activity [Elektronische Daten] [D. Morris, S. Stock, J. McLachlan] Normal and pathological formation of blood vessels is of considerable interest both in terms of basic scientific processes and clinical applications. Angiogenic events in the adult are likely to represent persistence of developmental mechanisms, and embryos are therefore a suitable experimental model for these processes. Among embryonic tissues, muscle is particularly appropriate for investigation, since it is highly vascularised from early stages. There are a number of competing explanations of how this process is controlled. Bioassays offer advantages over conventional molecular localisation techniques, in that they reveal the presence of active processed forms of the molecules under study, rather than non-processed forms, or non-translated meassages. Using these techniques, we report here that embryonic chick muscle, taken from the stages at which blood vessels are forming, produces an angiogenic activity on the chick chorioallantoic membrane (CAM), and transforms NR6 cells in soft agar. Basic fibroblast growth factor (bFGF) is shown to be angiogenic on the CAM in the same way, and also transforms NR6 cells (NR6 cells lack functional epidermal growth factor/transforming growth factor-a receptors, and are believed to respond only to bFGF in this way). Anti-bFGF removes the transforming activity of the embryonic muscle. We conclude that this represents evidence that embryonic chick muscle is producing an FGF-like molecule which is capable of acting as an angiogenic agent at the appropriate times in development. Birkhäuser Verlag Basel, 1996 Vasculogenesis nationallicence angiogenesis nationallicence muscle nationallicence embryonic nationallicence metabolites nationallicence fibroblast growth factor (FGF) nationallicence chorioallantoic membrane (CAM) nationallicence CAM : chorioallantoic membrane nationallicence FGF : fibroblast growth factor nationallicence aFGF : acidic fibroblast growth factor nationallicence bFGF : basic fibroblast growth factor nationallicence EGF : epidermal growth factor nationallicence TGF : transforming growth factor nationallicence VEGF : vascular endothelial growth factor nationallicence MEM : minimum essential medium nationallicence PBS : phosphate buffered saline nationallicence V.D.I. : vascular density index nationallicence Morris D. School of Biological and Medical Sciences, Bute Medical Building, University of St. Andrews, KY 16 9TS, St. Andrews, Scotland, (UK) aut Stock S. School of Biological and Medical Sciences, Bute Medical Building, University of St. Andrews, KY 16 9TS, St. Andrews, Scotland, (UK) aut McLachlan J. School of Biological and Medical Sciences, Bute Medical Building, University of St. Andrews, KY 16 9TS, St. Andrews, Scotland, (UK) aut Experientia Birkhäuser-Verlag 52/8(1996-08-01), 757-761 0014-4754 52:8<757 1996 52 18 https://doi.org/10.1007/BF01923985 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01923985 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morris D. School of Biological and Medical Sciences, Bute Medical Building, University of St. Andrews, KY 16 9TS, St. Andrews, Scotland, (UK) aut NATIONALLICENCE 700 1- Stock S. School of Biological and Medical Sciences, Bute Medical Building, University of St. Andrews, KY 16 9TS, St. Andrews, Scotland, (UK) aut NATIONALLICENCE 700 1- McLachlan J. School of Biological and Medical Sciences, Bute Medical Building, University of St. Andrews, KY 16 9TS, St. Andrews, Scotland, (UK) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/8(1996-08-01), 757-761 0014-4754 52:8<757 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer