caa a22 4500 477084109 CHVBK 20180405111458.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF01923990 doi (NATIONALLICENCE)springer-10.1007/BF01923990 Tissue protection against oxidative stress [Elektronische Daten] [S. Di Meo, P. Venditti, T. De Leo] We used an enhanced luminescence technique to study the response of rat tissues, such as liver, heart, muscle and blood, to oxidative stress and to determine their antioxidant capacity. As previously found for liver homogenate, the intensity of light emission (E) of tissue homogenates and blood samples, stressed with sodium perborate, is dependent on concentration, and the dose-response curves can be described by the equation E=a·C/exp(b·C). Theb value depends on the antioxidant defence capability of the tissues. In fact, it increases when homogenates are supplemented with an antioxidant, and is correlated with tissue antioxidant capacity, evaluated by two previously set up methods both using the same luminescence technique. Our results indicate that the order of antioxidant capacity of the tissues is liver>blood>heart>muscle. Thea value depends on the systems catalysing the production of radical species. In fact, it is related to the tissue level of hemoproteins, which are known to act as catalysts in radical production from hydroperoxides. The equation proposed to describe the dose-response relation is simple to handle and permits an immediate connection with the two characteristics of the systems analysed which determine their response to the pro-oxidant treatment. However, the equation which best describes the above relation for all the tissues is the following: E=α·C/exp(β·Cδ). The parameter δ assumes values smaller than 1, which seem to depend on relative amounts of tissue hemoproteins and antioxidants. The extension of the analysis to mitochondria shows that they respond to oxidative stress in a way analogous to the tissues, and that the adherence of the dose-response curve to the course predicted from the equation E=a·C/exp(b·C) is again dependent on hemoprotein content. Birkhäuser Verlag Basel, 1996 Oxygen radicals nationallicence enhanced luminescence nationallicence tissue antioxidants nationallicence oxidative stress nationallicence Di Meo S. Dipartimento di Fisiologia Generale ed Ambientale dell' Università di Napoli, V. Mezzocannone 8, I-80134, Napoli, (Italy) aut Venditti P. Dipartimento di Fisiologia Generale ed Ambientale dell' Università di Napoli, V. Mezzocannone 8, I-80134, Napoli, (Italy) aut De Leo T. Dipartimento di Fisiologia Generale ed Ambientale dell' Università di Napoli, V. Mezzocannone 8, I-80134, Napoli, (Italy) aut Experientia Birkhäuser-Verlag 52/8(1996-08-01), 786-794 0014-4754 52:8<786 1996 52 18 https://doi.org/10.1007/BF01923990 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01923990 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Di Meo S. Dipartimento di Fisiologia Generale ed Ambientale dell' Università di Napoli, V. Mezzocannone 8, I-80134, Napoli, (Italy) aut NATIONALLICENCE 700 1- Venditti P. Dipartimento di Fisiologia Generale ed Ambientale dell' Università di Napoli, V. Mezzocannone 8, I-80134, Napoli, (Italy) aut NATIONALLICENCE 700 1- De Leo T. Dipartimento di Fisiologia Generale ed Ambientale dell' Università di Napoli, V. Mezzocannone 8, I-80134, Napoli, (Italy) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/8(1996-08-01), 786-794 0014-4754 52:8<786 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer