caa a22 4500 477084176 CHVBK 20180405111458.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF01919320 doi (NATIONALLICENCE)springer-10.1007/BF01919320 Adenylate storage, metabolism and utilization in coelomic cells of the polychaete Nereis virens (Annelida, polychaeta) [Elektronische Daten] [U. Hoeger, C. Märker, G. Geier] Eleocytes are specialized coelomic cells in nereid annelids which assume a central role during germ cell development. They may contain extremely high concentrations of both adenosine monophosphate (AMP) and adenosine diphosphate (ADP) (each >10 μmol/ml of cell vol.), whereas the adenosine triphosphate (ATP) content is comparatively low (0.8 μmol/ml cell vol.).31P nuclear magnetic, resonance (NMR) studies of living eleocytes suggest the compartmentalization of both AMP and ADP in the large acidic vacuole characteristic for this cell type. Eleocytes are thus capable of storing high concentrations of ADP and AMP without inhibiting energy metabolism, by sequestering these compounds in a separate compartment. The high concentrations of both AMP and ADP in the eleocytes decrease in both males and females during the course of maturation. In eleocytes of male animals, the decline of the high nucleotide concentrations was accompanied by a transient increase of two intracellular nucleosides, inosine and guanosine. This suggests the degradation and further metabolism of nucleotides to the corresponding nucleosides. In culture, eleocytes release both inosine and guanosine into the medium. Both nucleosides are also present in the coelomic fluid, the common compartment for both eleocytes and germ cells. Both male and female germ cells incorporate14C-labelled inosine and guanosine in culture. For oocytes, the further incorporation of [14C]inosine into the RNA fraction could be demonstrated. The large adenylate pools in the eleocytes may be regarded as a store for purine compounds for later use by the growing germ cells to supplement nucleic acid synthesis. The supply of nucleic acid precursors seems to be another specific function of eleocytes related to gametogenesis, in addition to their known synthesis of vitellogenin. Birkhäuser Verlag, 1996 Polychaete nationallicence coelomic cells nationallicence eleocytes nationallicence adenylates nationallicence ADP nationallicence AMP nationallicence inosine nationallicence Hoeger U. Institut für Zoologie, Johannes-Gutenberg-Universität, Saarstrasse 21, D-55099, Mainz, (Germany) aut Märker C. Institut für Zoologie, Johannes-Gutenberg-Universität, Saarstrasse 21, D-55099, Mainz, (Germany) aut Geier G. Institut für Zoologie, Johannes-Gutenberg-Universität, Saarstrasse 21, D-55099, Mainz, (Germany) aut Experientia Birkhäuser-Verlag 52/5(1996-05-01), 481-486 0014-4754 52:5<481 1996 52 18 https://doi.org/10.1007/BF01919320 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01919320 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hoeger U. Institut für Zoologie, Johannes-Gutenberg-Universität, Saarstrasse 21, D-55099, Mainz, (Germany) aut NATIONALLICENCE 700 1- Märker C. Institut für Zoologie, Johannes-Gutenberg-Universität, Saarstrasse 21, D-55099, Mainz, (Germany) aut NATIONALLICENCE 700 1- Geier G. Institut für Zoologie, Johannes-Gutenberg-Universität, Saarstrasse 21, D-55099, Mainz, (Germany) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/5(1996-05-01), 481-486 0014-4754 52:5<481 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer