caa a22 4500 477084230 CHVBK 20180405111458.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF01919316 doi (NATIONALLICENCE)springer-10.1007/BF01919316 Mapping and quantification of biomolecules in tumor biopsies using bioluminescence [Elektronische Daten] [G. Schwickert, S. Walenta, W. Mueller-Klieser] Quantitative bioluminescence and single-photon imaging have been applied for mapping concentration distributions of metabolites, such as adenosine triphosphate (ATP), glucose and lactate, in biopsies of cervical cancers in patients. Biopsies were taken before a conventional radiation treatment, and a number of clinically relevant data, such as local tumor control, patient survival, metastatic spread and so forth, were documented. There was no correlation between staging or grading and any of the metabolic parameters measured. Local correlations between ATP, glucose and lactate on a pixel-to-pixel basis were generally positive, with respective Spearman's correlation coefficients less in patients without clinically documented metastasis compared with those with metastatic spread. Lactate concentrations were significantly higher and scattered over a wider range in tumors with metastatic spread in comparison to malignancies in patients without metastasis. Thus, high local lactate levels of ≥20 μmole/g appear to be associated with a high risk of metastasis, at least in human cervical tumors. Birkhäuser Verlag, 1996 Metabolic imaging nationallicence bioluminescence nationallicence single-photon imaging nationallicence human tumor biopsies nationallicence Schwickert G. Institute of Physiology & Pathophysiology, University of Mainz, Duesbergweg 6, D-55099, Mainz, Germany aut Walenta S. Institute of Physiology & Pathophysiology, University of Mainz, Duesbergweg 6, D-55099, Mainz, Germany aut Mueller-Klieser W. Institute of Physiology & Pathophysiology, University of Mainz, Duesbergweg 6, D-55099, Mainz, Germany aut Experientia Birkhäuser-Verlag 52/5(1996-05-01), 460-463 0014-4754 52:5<460 1996 52 18 https://doi.org/10.1007/BF01919316 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01919316 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schwickert G. Institute of Physiology & Pathophysiology, University of Mainz, Duesbergweg 6, D-55099, Mainz, Germany aut NATIONALLICENCE 700 1- Walenta S. Institute of Physiology & Pathophysiology, University of Mainz, Duesbergweg 6, D-55099, Mainz, Germany aut NATIONALLICENCE 700 1- Mueller-Klieser W. Institute of Physiology & Pathophysiology, University of Mainz, Duesbergweg 6, D-55099, Mainz, Germany aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/5(1996-05-01), 460-463 0014-4754 52:5<460 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer