caa a22 4500 477084354 CHVBK 20180405111459.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF01923356 doi (NATIONALLICENCE)springer-10.1007/BF01923356 Urinary excretion of renal brush border membrane enzymes in leprosy patientseffect of multidrug therapy [Elektronische Daten] [M. Kohli, N. Ganguly, S. Kaur, V. Sharma] Renal function at the brush border membrane level has been studied using characteristic enzymes, such as alkaline phosphatase, leucine-aminopeptidase and gamma-glutamyl transpeptidase. Urinary enzyme studies were performed using leprosy patients, classified on the basis of bacteriological index (BI>3; n=20,BI<3; n=12, BI-ve; n=10) and compared with control subjects (n=10). The role of enzymuria in monitoring WHO-recommended multidrug therapy (MDT) has been evaluated in these patients. A significant increase in the enzyme activities (p<0.01), as well as significant (p<0.01) proteinurea in 24-hour urine samples of both the smear positive groups (BI>3,BI<3) prior to therapy compared to control subjects, indicates proximal tubular functional impairment at brush border membrane level. In the smear negative (BI-ve) grou, no significant difference was observed in enzyme activities as compared with the control group. In a follow-up study (BI>3; n=13, BI<3; n=4) the activities of all the enzymes decreased significantly in all the groups when compared to a corresponding untreated group. The follow-up study was not carried out on the smear negative group. The surprising finding was the differential behaviour of r-glutamyl transpeptidase, whose activity increased significantly (p<0.01) even after therapy inBI>3 group when compared with untreated patients. However in a detailed work-up including hepatic and renal function tests, the serum biochemistry was found to be normal both before and after therapy. Urinary excretion of brush border enzymes seems to be related to bacterial load, and their potential in studying the effect of MDT remains unclear. Birkhäuser Verlag Basel, 1996 Alkaline phosphatase nationallicence leucine-aminopeptidase nationallicence gamma-glutamyl transpeptidase nationallicence bacteriological index nationallicence multidrug therapy nationallicence leprosy nationallicence Kohli M. Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, 160 012, Chandigarh, (India) aut Ganguly N. Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, 160 012, Chandigarh, (India) aut Kaur S. Department of Dermatology, Postgaraduate Institute of Medical Education and Research, 160 012, Chandigarh, (India) aut Sharma V. Department of Dermatology, Postgaraduate Institute of Medical Education and Research, 160 012, Chandigarh, (India) aut Experientia Birkhäuser-Verlag 52/2(1996-02-01), 127-130 0014-4754 52:2<127 1996 52 18 https://doi.org/10.1007/BF01923356 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01923356 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kohli M. Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, 160 012, Chandigarh, (India) aut NATIONALLICENCE 700 1- Ganguly N. Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, 160 012, Chandigarh, (India) aut NATIONALLICENCE 700 1- Kaur S. Department of Dermatology, Postgaraduate Institute of Medical Education and Research, 160 012, Chandigarh, (India) aut NATIONALLICENCE 700 1- Sharma V. Department of Dermatology, Postgaraduate Institute of Medical Education and Research, 160 012, Chandigarh, (India) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/2(1996-02-01), 127-130 0014-4754 52:2<127 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer