caa a22 4500 477084370 CHVBK 20180405111459.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF01923361 doi (NATIONALLICENCE)springer-10.1007/BF01923361 Watanabe T. Department of Hygiene and Preventive Medicine, Yamagata University School of Medicine, 2-2-2 Iida Nishi, 99023, Yamagata, (Japan) aut Morphological and biochemical effects of excessive amounts of biotin on embryonic development in mice [Elektronische Daten] [T. Watanabe] Pregnant mice received excessive amounts of biotin either subcutaneously (sc) or orally during gestation. There were no differences in the successful pregnancy rates and number of dead or resorbed fetuses between the control and biotin-treated groups. In biotin-treated groups no increased incidence of fetuses with external malformations was clearly demonstrable. However, biotin accumulated in maternal and embryonic organs; especially, the serum biotin level in the biotin-treated dam was 200-fold higher than that in the control dam. There was a difference in biotinidase activity in maternal serum and placenta between the control and biotin-treated groups. It was concluded that excessive amounts of biotin affected the specific activity of biotinidase in pregnant mice, but did not disturb normal reproductive functions and embryonic development. Birkhäuser Verlag Basel, 1996 Biotin excess nationallicence embryonic development nationallicence reproductive function nationallicence biotinidase nationallicence estradiol nationallicence Experientia Birkhäuser-Verlag 52/2(1996-02-01), 149-154 0014-4754 52:2<149 1996 52 18 https://doi.org/10.1007/BF01923361 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01923361 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Watanabe T. Department of Hygiene and Preventive Medicine, Yamagata University School of Medicine, 2-2-2 Iida Nishi, 99023, Yamagata, (Japan) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/2(1996-02-01), 149-154 0014-4754 52:2<149 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer