caa a22 4500 477084400 CHVBK 20180405111459.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF01923359 doi (NATIONALLICENCE)springer-10.1007/BF01923359 A simple, sensitive, non-stimulated photon counting system for detection of superoxide anion in whole blood [Elektronische Daten] [F. Lu, J. Lin, H. Wang, W. Huang] A simple, sensitive, non-stimulated assay was developed to measure the superoxide anion concentration in whole blood, using an ultra-sensitive chemiluminescence (CL) analyzer, and lucignin amplification. The assay system can be performed without leukocyte isolation or stimulant administration. The blood CL levels of healthy males (362.8±337.7 counts/10 sec) were not different from those of females (335±308.7 counts/10 sec) (p=0.64), whereas the CL levels in whole blood in patients with acute pancreatitis (2522±2014 counts/10 sec) were significantly higher than those of healthy controls (p<0.001). This assay system may be valuable in the future for quantitative measurement of reactive oxygen species in various disorders. Birkhäuser Verlag Basel, 1996 Superoxide nationallicence free radical nationallicence reactive oxygen species nationallicence chemiluminescence nationallicence pancreatitis nationallicence ROS : reactive oxygen species nationallicence CL : chemiluminescence nationallicence PMA : phorbol myristate acetate nationallicence SOD : superoxide dismutase nationallicence WBC : white blood cells nationallicence PMNL : polymorphonuclear leukocytes nationallicence Lu F. Departments of Biochemistry College of Medicine, National Taiwan University, Taipei, Taiwan (Republic of China) aut Lin J. Departments of Internal Medicine College of Medicine, National Taiwan University, Taipei, Taiwan (Republic of China) aut Wang H. Departments of Emergency, College of Medicine, National Taiwan University, Taipei, Taiwan (Republic of China) aut Huang W. Departments of Biochemistry College of Medicine, National Taiwan University, Taipei, Taiwan (Republic of China) aut Experientia Birkhäuser-Verlag 52/2(1996-02-01), 141-144 0014-4754 52:2<141 1996 52 18 https://doi.org/10.1007/BF01923359 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01923359 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu F. Departments of Biochemistry College of Medicine, National Taiwan University, Taipei, Taiwan (Republic of China) aut NATIONALLICENCE 700 1- Lin J. Departments of Internal Medicine College of Medicine, National Taiwan University, Taipei, Taiwan (Republic of China) aut NATIONALLICENCE 700 1- Wang H. Departments of Emergency, College of Medicine, National Taiwan University, Taipei, Taiwan (Republic of China) aut NATIONALLICENCE 700 1- Huang W. Departments of Biochemistry College of Medicine, National Taiwan University, Taipei, Taiwan (Republic of China) aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/2(1996-02-01), 141-144 0014-4754 52:2<141 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer