caa a22 4500 477084435 CHVBK 20180405111459.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF01923353 doi (NATIONALLICENCE)springer-10.1007/BF01923353 Effect of nonprotein thiols on protein synthesis in isolated rat hepatocytes [Elektronische Daten] [M. Asensi, A. Carcía-España, F. Pallardó, J. Viña, J. Estrela] The ability of nonprotein thiols to modulate rates of protein synthesis was investigated in isolated rat hepatocytes. Addition of cysteine stimulates protein labelling by [14C] Leucine. Glutahione depletion, induced by in vivod administration of L-buthionine sulfoximine and diethylmaleate, did not alter the effect of cysteine, although it decreased the rate of protein synthesis by 32%. The effect of cysteine on protein synthesis does not seem to be related to a perturbatin of the redox state of the NAD+/NADH system or to changes in the rate of gluconeogenic pathway. The following observations indicate that cysteine may stimulate protein syntheis by increasing intracellular levels of aspartate: 1. Amino-oxyacetate, an inhibitor of pyridoxyal-dependent enzymes, inhibits protein labelling and decreases aspartate cellular content, whereas most amino acids accumulate or remain unchanged; 2. Cysteine, in the absence or in the presence of amino-ocycetate, stimulates protein labelling and induces aspartate accumulation, although mot amino acids diminish or remain unchanged. Birkhäuser Verlag Basel, 1996 Cysteine nationallicence glutathione nationallicence protein synthesis nationallicence aspartate nationallicence thiols nationallicence GSH : reduced glutathione nationallicence GSSG : oxidized glutathione nationallicence GSH ester : glutathione monoethyl ester nationallicence BSO : L-buthionine sulfoximine nationallicence DEM : diethylmaleate nationallicence NAC : N-acetyl cysteine nationallicence AOA : amino-oxyacetate nationallicence Asensi M. Department de Fisiología, Facultad de Medicina, Avda. Blasco Ibañe 17, E-46010, Valencia aut Carcía-España A. Departmento de Bioquímica y Biología Molecular, Universidad de Valencia, Valencia, (Spain) aut Pallardó F. Department de Fisiología, Facultad de Medicina, Avda. Blasco Ibañe 17, E-46010, Valencia aut Viña J. Department de Fisiología, Facultad de Medicina, Avda. Blasco Ibañe 17, E-46010, Valencia aut Estrela J. Department de Fisiología, Facultad de Medicina, Avda. Blasco Ibañe 17, E-46010, Valencia aut Experientia Birkhäuser-Verlag 52/2(1996-02-01), 111-114 0014-4754 52:2<111 1996 52 18 https://doi.org/10.1007/BF01923353 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01923353 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Asensi M. Department de Fisiología, Facultad de Medicina, Avda. Blasco Ibañe 17, E-46010, Valencia aut NATIONALLICENCE 700 1- Carcía-España A. Departmento de Bioquímica y Biología Molecular, Universidad de Valencia, Valencia, (Spain) aut NATIONALLICENCE 700 1- Pallardó F. Department de Fisiología, Facultad de Medicina, Avda. Blasco Ibañe 17, E-46010, Valencia aut NATIONALLICENCE 700 1- Viña J. Department de Fisiología, Facultad de Medicina, Avda. Blasco Ibañe 17, E-46010, Valencia aut NATIONALLICENCE 700 1- Estrela J. Department de Fisiología, Facultad de Medicina, Avda. Blasco Ibañe 17, E-46010, Valencia aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/2(1996-02-01), 111-114 0014-4754 52:2<111 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer