caa a22 4500 477084478 CHVBK 20180405111500.0 cr unu---uuuuu 170330e19960201xx s 000 0 eng 10.1007/BF01923351 doi (NATIONALLICENCE)springer-10.1007/BF01923351 Asscorbic acid portects against male infertility in a teleost fish [Elektronische Daten] [K. Dabrowski, A. Ciereszko] An animal unable to synthesize ascorbic acid uniquely minicks human and non-human primates. Therefore, in this study we used the rainbow trout, a teleost fish, as the model animal to study the importance of dietary ascorbic acid on the fertilizing ability of sperm. A high concentration of ascorbic acid in semen plays a key role in maintaining the genetic integrity of sperm cells, by preventing oxidative damage to sperm DNA. This study will show that the concentration of asorbic acid in seminal plasma refelcts the dietary fed either an ascorbate-free diet (from 4.74±0.9 to 0.16±0.08 μg ml−1) or an ascorbate-rich diet (from 37.9±4.7 to 17.7± 3.2 μg ml−1) during the sperimnation season. The relationship between ascrobate status and fertility was studied in six groups of fish fed graded levels of ascorbic acid, which sperimated over a 150-day-period. Sperm from individual males was used to fertilize several batches of eggs. When the seminal plasma ascorbate concentration decreased to 7.3 μg ml−1 a significant decrease of fertilization rate and the hatching rate of embryos resulted. This is the first evidence that dietary ascorbate level directly affected sperm quality and influenced male fertility in a scruvy-prone vertebrate. Birkhäuser Verlag Basel, 1996 Vitamin C nationallicence spermatozoa nationallicence ascorbate-deficient nationallicence salmonid nationallicence Dabrowski K. School of Natural Resources, The Ohio State University, 43210, Columbus, Ohio, USA aut Ciereszko A. School of Natural Resources, The Ohio State University, 43210, Columbus, Ohio, USA aut Experientia Birkhäuser-Verlag 52/2(1996-02-01), 97-100 0014-4754 52:2<97 1996 52 18 https://doi.org/10.1007/BF01923351 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF01923351 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dabrowski K. School of Natural Resources, The Ohio State University, 43210, Columbus, Ohio, USA aut NATIONALLICENCE 700 1- Ciereszko A. School of Natural Resources, The Ohio State University, 43210, Columbus, Ohio, USA aut NATIONALLICENCE 773 0- Experientia Birkhäuser-Verlag 52/2(1996-02-01), 97-100 0014-4754 52:2<97 1996 52 18 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer