caa a22 4500 47708656X CHVBK 20180405111504.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00171136 doi (NATIONALLICENCE)springer-10.1007/BF00171136 Human keratinocyte culture from the peritonsillar mucosa [Elektronische Daten] [P. Neugebauer, B. Bonnekoh, A. Wevers, O. Michel, G. Mahrle, T. Krieg, E. Stennert] Tonsillectomy tissue can be used as a routine source for cultures of oropharyngeal keratinocytes. In so doing, a peritonsillar strip of unaltered mucosa was dissected in the upper submucosa. Subsequent trypsinization yielded 7.0 \+- 3.4 × 106 keratinocytes per bilateral tonsillectomy. Keratinocyte attachment and growth in primary culture were promoted by sublethally irradiated 3T3 murine fibroblasts. Three subcultures could be performed without a feeder layer and were characterized by a population doubling time of 4.5 days during log growth phase. Electrophoretic and immunoblot analysis of the third subculture revealed a strong expression of keratin pairs 5/14 and 6/16 as well as keratins 7 and 19, whereas keratins 8/18 were expressed less intensely. The lowest intensity was found for keratin 13, which is known to be indicative of the differentiated mucosa. The culture technique thus provides an easily available in vitro model for morphological and functional studies on the epithelial compartment of human oropharyngeal mucosa. Springer-Verlag, 1996 Tonsillectomy nationallicence Oral mucosa nationallicence Keratinocytes nationallicence Cell Culture nationallicence Keratin pattern nationallicence Neugebauer P. Hals-Nasen-Ohren-Klinik der Universität Köln, Joseph-Stelzmann-Strasse 9, D-50931, Cologne, Germany aut Bonnekoh B. Hautklinik der Universität Köln, Joseph-Stelzmann-Strasse 9, D-50931, Cologne, Germany aut Wevers A. Hautklinik der Universität Köln, Joseph-Stelzmann-Strasse 9, D-50931, Cologne, Germany aut Michel O. Hals-Nasen-Ohren-Klinik der Universität Köln, Joseph-Stelzmann-Strasse 9, D-50931, Cologne, Germany aut Mahrle G. Hautklinik der Universität Köln, Joseph-Stelzmann-Strasse 9, D-50931, Cologne, Germany aut Krieg T. Hautklinik der Universität Köln, Joseph-Stelzmann-Strasse 9, D-50931, Cologne, Germany aut Stennert E. Hals-Nasen-Ohren-Klinik der Universität Köln, Joseph-Stelzmann-Strasse 9, D-50931, Cologne, Germany aut European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/4-5(1996-05-01), 245-251 0937-4477 253:4-5<245 1996 253 405 https://doi.org/10.1007/BF00171136 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00171136 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Neugebauer P. Hals-Nasen-Ohren-Klinik der Universität Köln, Joseph-Stelzmann-Strasse 9, D-50931, Cologne, Germany aut NATIONALLICENCE 700 1- Bonnekoh B. Hautklinik der Universität Köln, Joseph-Stelzmann-Strasse 9, D-50931, Cologne, Germany aut NATIONALLICENCE 700 1- Wevers A. Hautklinik der Universität Köln, Joseph-Stelzmann-Strasse 9, D-50931, Cologne, Germany aut NATIONALLICENCE 700 1- Michel O. Hals-Nasen-Ohren-Klinik der Universität Köln, Joseph-Stelzmann-Strasse 9, D-50931, Cologne, Germany aut NATIONALLICENCE 700 1- Mahrle G. Hautklinik der Universität Köln, Joseph-Stelzmann-Strasse 9, D-50931, Cologne, Germany aut NATIONALLICENCE 700 1- Krieg T. Hautklinik der Universität Köln, Joseph-Stelzmann-Strasse 9, D-50931, Cologne, Germany aut NATIONALLICENCE 700 1- Stennert E. Hals-Nasen-Ohren-Klinik der Universität Köln, Joseph-Stelzmann-Strasse 9, D-50931, Cologne, Germany aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/4-5(1996-05-01), 245-251 0937-4477 253:4-5<245 1996 253 405 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer