caa a22 4500 477086586 CHVBK 20180405111504.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00171142 doi (NATIONALLICENCE)springer-10.1007/BF00171142 Immunohistochemical localization of manganese superoxide dismutase in the rat cochlea [Elektronische Daten] [M. Lai, T. Ohmichi, K. Egusa, S. Okada, Y. Masuda] There has been recent increasing interest in the involvement of superoxide radicals (O2 −) and their scavenging enzymes, the superoxide dismutases, in the pathophysiology of certain diseases. Since mitochondria are significant intracellular sources of O2 − and important targets of oxidant injury, determining the intracochlear localization of mitochondrial O2 − scavenging enzyme may provide important insight into the pathogenesis of injury due to cochlear oxidants. In order to locate the mitochondrial O2 − scavenging enzyme, manganese superoxide dismutase (MnSOD), the authors used a modified immunoglobulin peroxidase bridge sequence method to detect MnSOD in paraffin-embedded, formalin-fixed rat cochleas. Site-specific immunolocalization of MnSOD could be demonstrated in the cochlear labyrinth, suggesting that the generation of intracochlear O2 − was possibly implicated in the metabolically active sites and sites rich in vascularity. This study also provided a useful probe for detecting MnSOD immunohistochemically from ethylenediamine tetraacetic acid-treated materials without requiring an antigen retrieval procedure. Springer-Verlag, 1996 Superoxide radicals nationallicence Manganese superoxide dismutase nationallicence Immunohistochemical method nationallicence Cochlear labyrinth nationallicence Lai M. Department of Otolaryngology, Okayama University Medical School, 2-5-1 Shikata-Cho, 700, Okayama, Japan aut Ohmichi T. Department of Otolaryngology, Okayama University Medical School, 2-5-1 Shikata-Cho, 700, Okayama, Japan aut Egusa K. Department of Otolaryngology, Okayama University Medical School, 2-5-1 Shikata-Cho, 700, Okayama, Japan aut Okada S. Department of Pathology, Okayama University Medical School, Japan aut Masuda Y. Department of Otolaryngology, Okayama University Medical School, 2-5-1 Shikata-Cho, 700, Okayama, Japan aut European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/4-5(1996-05-01), 273-277 0937-4477 253:4-5<273 1996 253 405 https://doi.org/10.1007/BF00171142 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00171142 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lai M. Department of Otolaryngology, Okayama University Medical School, 2-5-1 Shikata-Cho, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Ohmichi T. Department of Otolaryngology, Okayama University Medical School, 2-5-1 Shikata-Cho, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Egusa K. Department of Otolaryngology, Okayama University Medical School, 2-5-1 Shikata-Cho, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Okada S. Department of Pathology, Okayama University Medical School, Japan aut NATIONALLICENCE 700 1- Masuda Y. Department of Otolaryngology, Okayama University Medical School, 2-5-1 Shikata-Cho, 700, Okayama, Japan aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/4-5(1996-05-01), 273-277 0937-4477 253:4-5<273 1996 253 405 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer