caa a22 4500 477086683 CHVBK 20180405111505.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00171137 doi (NATIONALLICENCE)springer-10.1007/BF00171137 Interleukin 1 (IL-1) and IL-1-receptor antagonist (IL-1-RA) in middle ear cholesteatoma: an analysis of protein production and biological activity [Elektronische Daten] [J. Bujía, C. Kim, P. Ostos, H. Sudhoff, E. Kastenbauer, L. Hültner] Cytokine networks are now presumed to play an essential role in the pathogenesis of middle ear cholesteatoma. Of the factors identified in cholesteatoma, interleukin-1 (IL-1)-alpha appears to be especially important because of its stimulation of keratinocyte proliferation as well induction of bone resorption. To further characterize the possible role of IL-1 in the pathogenesis of cholesteatoma, we quantified the levels of IL-1 and IL-1-receptor antagonist (IL-1-RA) present using the bicinchonic acid protein assay and enzyme-linked immunosorbent assay (ELISA) on tissue extracts from 20 cholesteatoma specimens. The presence of biologically active IL-1 was also analyzed, using the cell line LBRM-33 and an ELISA for the detection of interleukin-2 (IL-2). Human skin obtained from the external ear canal was used as control. The amounts of IL-1-alpha in cholesteatoma (34.9 \+-19.5) were higher than in human skin (6.7 \+- 2.8). The observed differences were statistically significant by Student's t-test (P < 0.01). Skin samples showed elevated concentrations of IL-1-RA (248.3 \+- 30.2) in comparison to that in the cholesteatoma (80.8 \+- 13.5). This was also statistically significant (P < 0.01). Whereas IL-1 activity was not detected in skin samples, all cholesteatoma specimens studied showed a stimulation effect on the production of IL-2 when incubated with the cell line LBRM-33. The results point to an over-expression of IL-1 concurrent with a decreased secretion of IL-1-RA in middle ear cholesteatoma. Furthermore IL-1-RA production is deficient relative to total IL-1 production, resulting in the presence of active IL-1. Springer-Verlag, 1996 Middle ear cholesteatoma nationallicence Interleukin-1 nationallicence Interleukin-l-receptor antagonist nationallicence Histochemistry nationallicence Bioassay nationallicence Bujía J. Department of Otolaryngology, Head-Neck Surgery, Ludwig Maximilians University, Klinikum Großhadem, Marchioninistrasse 15, D-81366, Munich, Germany aut Kim C. Department of Otolaryngology, Head-Neck Surgery, Ludwig Maximilians University, Klinikum Großhadem, Marchioninistrasse 15, D-81366, Munich, Germany aut Ostos P. Department of Otolaryngology, Head-Neck Surgery, University of Cordoba, Cordoba, Spain aut Sudhoff H. Department of Otorhinolaryngology, St. Elisabeth Hospital, University of Bochum, Bochum, Germany aut Kastenbauer E. Department of Otolaryngology, Head-Neck Surgery, Ludwig Maximilians University, Klinikum Großhadem, Marchioninistrasse 15, D-81366, Munich, Germany aut Hültner L. GSF, Institute for Experimental Hematology, Munich, Germany aut European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/4-5(1996-05-01), 252-255 0937-4477 253:4-5<252 1996 253 405 https://doi.org/10.1007/BF00171137 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00171137 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bujía J. Department of Otolaryngology, Head-Neck Surgery, Ludwig Maximilians University, Klinikum Großhadem, Marchioninistrasse 15, D-81366, Munich, Germany aut NATIONALLICENCE 700 1- Kim C. Department of Otolaryngology, Head-Neck Surgery, Ludwig Maximilians University, Klinikum Großhadem, Marchioninistrasse 15, D-81366, Munich, Germany aut NATIONALLICENCE 700 1- Ostos P. Department of Otolaryngology, Head-Neck Surgery, University of Cordoba, Cordoba, Spain aut NATIONALLICENCE 700 1- Sudhoff H. Department of Otorhinolaryngology, St. Elisabeth Hospital, University of Bochum, Bochum, Germany aut NATIONALLICENCE 700 1- Kastenbauer E. Department of Otolaryngology, Head-Neck Surgery, Ludwig Maximilians University, Klinikum Großhadem, Marchioninistrasse 15, D-81366, Munich, Germany aut NATIONALLICENCE 700 1- Hültner L. GSF, Institute for Experimental Hematology, Munich, Germany aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/4-5(1996-05-01), 252-255 0937-4477 253:4-5<252 1996 253 405 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer