caa a22 4500 47708673X CHVBK 20180405111505.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00171130 doi (NATIONALLICENCE)springer-10.1007/BF00171130 Morphological changes in rat submandibular gland mucous cells during fixation with 10% formalin [Elektronische Daten] [H. Kakoi, M. Anniko, C. Erwall, C. Pettersson, B. Jansson] The features of mucous cells in 10% formalin (FA)-fixed submandibular glands differ markedly from those fixed in glutaraldehyde (GA). We therefore studied morphological changes in mucous cells during 10% FA fixation. Mucous cells were fixed in either 10% FA, neutral sodium-phosphate-buffered (Na-PBed) 10% FA, ice-cold 10% FA or an ice-cold fixative mixture of 2.0% paraformaldehyde (PA) and 0.5% GA. Two different methods were used: immersion fixation and venous perfusion fixation. The 10% FA-fixed tissues had elliptical or flattened nuclei, a clear cytoplasm and no secretory granules. Tissues fixed with the fixative mixture displayed almost round nuclei, a broad endoplasmic reticulum and abundant secretory granules in the cytoplasm. Tissues immersion-fixed with neutral Na-PBed 10% FA or perfusion-fixed with ice-cold 10% FA had almost the same light microscopic appearance as that of the mixture-fixed tissues. To elucidate the process of morphological changes during 10% FA fixation at room temperature, samples immersed in 10% FA for varying periods of time were postfixed immediately in the fixative mixture and exposed to microwave irradiation. This method produced a variety of findings, even within the same section. There was a significant difference in the findings seen in the center of the section and at the periphery. The initial changes caused by 10% FA were rupture of the secretory granules located in the perinuclear region and destruction of the perinuclear organelles such as Golgi apparatus, mitochondria and endoplasmic reticulum. Absorption of the endoplasmic reticulum progressed so that the pennuclear region became translucent. To obtain a better structure in mucous cells from the fixed submandibular gland tissues, an appropriate fixative such as GA should be used and the fixative should infiltrate into the tissue as quickly as possible. Springer-Verlag, 1996 Formalin fixation nationallicence Mucous cell nationallicence Submandibular gland nationallicence Morphology nationallicence Rat nationallicence Kakoi H. Department of Otorhinolaryngology and Head & Neck Surgery, University Hospital (Akademiska sjukhuset), S-751 85, Uppsala, Sweden aut Anniko M. Department of Otorhinolaryngology and Head & Neck Surgery, University Hospital (Akademiska sjukhuset), S-751 85, Uppsala, Sweden aut Erwall C. Department of Otorhinolaryngology and Head & Neck Surgery, University Hospital (Akademiska sjukhuset), S-751 85, Uppsala, Sweden aut Pettersson C. Department of Otorhinolaryngology and Head & Neck Surgery, University Hospital (Akademiska sjukhuset), S-751 85, Uppsala, Sweden aut Jansson B. Department of Otorhinolaryngology and Head & Neck Surgery, University Hospital (Akademiska sjukhuset), S-751 85, Uppsala, Sweden aut European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/4-5(1996-05-01), 214-221 0937-4477 253:4-5<214 1996 253 405 https://doi.org/10.1007/BF00171130 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00171130 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kakoi H. Department of Otorhinolaryngology and Head & Neck Surgery, University Hospital (Akademiska sjukhuset), S-751 85, Uppsala, Sweden aut NATIONALLICENCE 700 1- Anniko M. Department of Otorhinolaryngology and Head & Neck Surgery, University Hospital (Akademiska sjukhuset), S-751 85, Uppsala, Sweden aut NATIONALLICENCE 700 1- Erwall C. Department of Otorhinolaryngology and Head & Neck Surgery, University Hospital (Akademiska sjukhuset), S-751 85, Uppsala, Sweden aut NATIONALLICENCE 700 1- Pettersson C. Department of Otorhinolaryngology and Head & Neck Surgery, University Hospital (Akademiska sjukhuset), S-751 85, Uppsala, Sweden aut NATIONALLICENCE 700 1- Jansson B. Department of Otorhinolaryngology and Head & Neck Surgery, University Hospital (Akademiska sjukhuset), S-751 85, Uppsala, Sweden aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/4-5(1996-05-01), 214-221 0937-4477 253:4-5<214 1996 253 405 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer