caa a22 4500 477086748 CHVBK 20180405111505.0 cr unu---uuuuu 170330e19960501xx s 000 0 eng 10.1007/BF00171143 doi (NATIONALLICENCE)springer-10.1007/BF00171143 Application of the ATP-bioluminescence assay to thermosensitivity testing for head and neck cancer [Elektronische Daten] [T. Ohtsubo, H. Saito, N. Tanaka, E. Kano] We investigated the usefulness of the ATP assay as a thermosensitivity test in comparison with a colony-forming assay (CFA). The intracellular ATP levels in KB cells were markedly decreased after exposure to hyperthermia (43°C for 1 h), reaching less than half that in the control cells in 12-18 h, and recovering gradually thereafter. The effect of hyperthermia assessed by the ATP assay closely correlated to that assessed by CFA, not only when KB cells were heated at 42, 43, and 44°C but also in the conditions where L, KB and IMC-3 cell lines were heated at 43°C. Findings showed that the ATP assay can be performed easily and more quickly than CFA for evaluating cell viability after heating. We also investigated heterogeneous responses to hyperthermia in cells from fresh surgical specimens of tumors. Thus, this rapid and reliable test was found to be useful for predicting the outcome of hyperthermia as a clinical treatment. Springer-Verlag, 1996 Head and neck cancer nationallicence Thermosensitivity nationallicence ATP assay nationallicence Ohtsubo T. Department of Otolaryngology, Fukui Medical School, 23 Simo-aitsuku, 910-11, Matsuoka, Fukui, Japan aut Saito H. Department of Otolaryngology, Fukui Medical School, 23 Simo-aitsuku, 910-11, Matsuoka, Fukui, Japan aut Tanaka N. Department of Otolaryngology, Fukui Medical School, 23 Simo-aitsuku, 910-11, Matsuoka, Fukui, Japan aut Kano E. Department of Otolaryngology, Fukui Medical School, 23 Simo-aitsuku, 910-11, Matsuoka, Fukui, Japan aut European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/4-5(1996-05-01), 278-282 0937-4477 253:4-5<278 1996 253 405 https://doi.org/10.1007/BF00171143 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00171143 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ohtsubo T. Department of Otolaryngology, Fukui Medical School, 23 Simo-aitsuku, 910-11, Matsuoka, Fukui, Japan aut NATIONALLICENCE 700 1- Saito H. Department of Otolaryngology, Fukui Medical School, 23 Simo-aitsuku, 910-11, Matsuoka, Fukui, Japan aut NATIONALLICENCE 700 1- Tanaka N. Department of Otolaryngology, Fukui Medical School, 23 Simo-aitsuku, 910-11, Matsuoka, Fukui, Japan aut NATIONALLICENCE 700 1- Kano E. Department of Otolaryngology, Fukui Medical School, 23 Simo-aitsuku, 910-11, Matsuoka, Fukui, Japan aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/4-5(1996-05-01), 278-282 0937-4477 253:4-5<278 1996 253 405 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer