caa a22 4500 477086810 CHVBK 20180405111505.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF00178288 doi (NATIONALLICENCE)springer-10.1007/BF00178288 Effect of growth factors on matrix synthesis by human nasal chondrocytes cultured in monolayer and in agar [Elektronische Daten] [J. Bujía, P. Pitzke, E. Kastenbauer, E. Wilmes, C. Hammer] Reconstructive surgery of multiple areas of the body may require replacement bone or cartilage transplants to repair defects or lesions of skeletal tissue. Advances in cell and tissue culture techniques now permit synthesis of autologous human cartilage in vitro. Several growth factors regulate the metabolism and activation of cartilage cells. To enhance culture conditions and effectiveness for in vitro cartilage engineering, the aim of our investigations was to characterize the influence of transforming growth factor (TGF)-beta and basic fibroblast growth factor (bFGF) on human nasal septal chondrocytes. The isolated cells were cultured as monolayers on plastic and in soft agar. The biological effects of the growth factors were assessed by determining synthesis of total protein and proteoglycan. TGF-beta caused a dose-dependent stimulation of total protein as well as glycosaminoglycan synthesis by all chondrocytes cultured. This stimulatory effect of TGF-beta was greater for chondrocytes cultured in soft agar than for chondrocytes cultured on plastic. No stimulatory effects of matrix synthesis was observed for bFGF in either culture condition. Our results show that TGF-beta can be employed to enhance in vitro production of cartilage grafts for reconstructive surgery. Springer-Verlag, 1996 Transforming growth factor beta nationallicence Basic fibroblast growth factor nationallicence Chondrocyte nationallicence Cartilage grafting nationallicence In vitro synthesis nationallicence Bujía J. HNO-Klinik und Poliklinik, Klinikum Großhadern, Marchioninistrasse 15, D-81377, Munich, Germany aut Pitzke P. HNO-Klinik und Poliklinik, Klinikum Großhadern, Marchioninistrasse 15, D-81377, Munich, Germany aut Kastenbauer E. HNO-Klinik und Poliklinik, Klinikum Großhadern, Marchioninistrasse 15, D-81377, Munich, Germany aut Wilmes E. HNO-Klinik und Poliklinik, Klinikum Großhadern, Marchioninistrasse 15, D-81377, Munich, Germany aut Hammer C. Klinikum Großhadern, Institut für Chirurgische Forschung, Marchioninistrasse 15, D-81377, Munich, Germany aut European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/6(1996-08-01), 336-340 0937-4477 253:6<336 1996 253 405 https://doi.org/10.1007/BF00178288 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00178288 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bujía J. HNO-Klinik und Poliklinik, Klinikum Großhadern, Marchioninistrasse 15, D-81377, Munich, Germany aut NATIONALLICENCE 700 1- Pitzke P. HNO-Klinik und Poliklinik, Klinikum Großhadern, Marchioninistrasse 15, D-81377, Munich, Germany aut NATIONALLICENCE 700 1- Kastenbauer E. HNO-Klinik und Poliklinik, Klinikum Großhadern, Marchioninistrasse 15, D-81377, Munich, Germany aut NATIONALLICENCE 700 1- Wilmes E. HNO-Klinik und Poliklinik, Klinikum Großhadern, Marchioninistrasse 15, D-81377, Munich, Germany aut NATIONALLICENCE 700 1- Hammer C. Klinikum Großhadern, Institut für Chirurgische Forschung, Marchioninistrasse 15, D-81377, Munich, Germany aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/6(1996-08-01), 336-340 0937-4477 253:6<336 1996 253 405 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer