caa a22 4500 47708690X CHVBK 20180405111505.0 cr unu---uuuuu 170330e19960801xx s 000 0 eng 10.1007/BF00178289 doi (NATIONALLICENCE)springer-10.1007/BF00178289 Antiproliferative effects of the biologically active metabolite of vitamin D3 (1,25 [OH]2 D3) on head and neck squamous cell carcinoma cell lines [Elektronische Daten] [J. Kornfehl, M. Formanek, A. Temmel, B. Knerer, M. Willheim] In addition to a role in calcium and phosphate homeostasis other vitamin D receptor (VDR) mediated effects have been discovered during the past few years for the biologically active metabolite of vitamin D, 1,25(OH)2D3. An antiproliferative, differentiation-inducing effect on nonmalignant and neoplastic cells of different origin has now been described. We examined the influence of 1,25(OH)2D3 on human squamous cell carcinomas of the head and neck (SCCHN). A differentiated (JP-PA) and undifferentiated (LF-FR) SCCHN line was studied with respect to proliferative capacity (using [3H]-thymidine uptake and cell number) and cell cycle distribution as determined by flow cytometry (FACS). Both cell lines were positive for VDR, which was found to be increased after the addition of 10−7 M1,25(OH)2D3, as shown by FACS analyses. The administration of 1,25(OH)2D3 at a concentration between 10−7 M and10−10 M caused a dose-dependent moderate growth inhibition, as reflected by down-regulation of DNA synthesis (reduced [3H]-thymidine uptake) and a decrease in cell numbers. The JP-PA cell line showed a significant growth reduction for both concentrations tested, whereas for LF-FR a significant inhibition was detected only for 10−7 M. The addition of 10−7 M 1,25(OH)2D3 caused a blockade in the transition of cells from G1 to S phase in both cell lines, with a significant accumulation of cells in the G0/G1 phase. Our results demonstrate a receptor-mediated, dose-dependent inhibition of neoplastic growth by 1,25(OH)2D3 in human SCCHN lines. Springer-Verlag, 1996 Vitamin D receptor nationallicence Cell cycle nationallicence Growth inhibition nationallicence Dose dependency nationallicence Kornfehl J. Department of Oto-Rhino-Laryngology, University of Vienna, Währinger Gürtel 18-20, A-1090, Vienna, Austria aut Formanek M. Department of Oto-Rhino-Laryngology, University of Vienna, Währinger Gürtel 18-20, A-1090, Vienna, Austria aut Temmel A. Department of Oto-Rhino-Laryngology, University of Vienna, Währinger Gürtel 18-20, A-1090, Vienna, Austria aut Knerer B. Department of Oto-Rhino-Laryngology, University of Vienna, Währinger Gürtel 18-20, A-1090, Vienna, Austria aut Willheim M. Institute of General and Experimental Pathology, University of Vienna, Vienna, Austria aut European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/6(1996-08-01), 341-344 0937-4477 253:6<341 1996 253 405 https://doi.org/10.1007/BF00178289 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00178289 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kornfehl J. Department of Oto-Rhino-Laryngology, University of Vienna, Währinger Gürtel 18-20, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Formanek M. Department of Oto-Rhino-Laryngology, University of Vienna, Währinger Gürtel 18-20, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Temmel A. Department of Oto-Rhino-Laryngology, University of Vienna, Währinger Gürtel 18-20, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Knerer B. Department of Oto-Rhino-Laryngology, University of Vienna, Währinger Gürtel 18-20, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Willheim M. Institute of General and Experimental Pathology, University of Vienna, Vienna, Austria aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/6(1996-08-01), 341-344 0937-4477 253:6<341 1996 253 405 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer