caa a22 4500 477086969 CHVBK 20180405111506.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00168492 doi (NATIONALLICENCE)springer-10.1007/BF00168492 Exposure of organ cultures from human tracheal epithelium to chemical carcinogens and subsequent long-term co-cultivation with autologous isotopic fibroblasts [Elektronische Daten] [I. Haas, P. Koldovsky, U. Ganzer] As a continuation of previous experiments introducing an extracorporeal model for transformation of human respiratory epithelium that might be able to mimic a spontaneously occurring malignant tumor, we prepared organ cultures from tracheal specimens and exposed them repeatedly to chemical carcinogens, using benzo(a)pyrene and methylnitronitrosoguanine for 6 weeks. We then tried to select possibly initiated cells by subsequent co-cultivation with autologous isotopic fibroblasts for 2 years. Nontreated controls were maintained from the same specimens and cultured in the same manner. By this technique we selected from specimen La24 three long-living cell lines with varying morphology and an antigenic pattern indicating dedifferentiation. The cells expressed simultaneously a panel of cytokeratins, vimentin and neuroectodermal antigens. Transplantation of these cell lines under the subrenal capsule of athymic mice resulted in tumor-like nodules of limited size. Success rate was dependent on time of previous in vitro culture and carcinogen treatment. None of the lines produced invasive or metastasizing tumors. Springer-Verlag, 1996 Organ cultures nationallicence Respiratory epithelium Chemical carcinogens nationallicence Cellular antigens nationallicence Mixed cultures nationallicence Haas I. Department of Otorhinolaryngology, Head and Neck Surgery, Heinrich Heine University, Moorenstrasse 5, D-40225, Düsseldorf, Germany aut Koldovsky P. Department of Otorhinolaryngology, Head and Neck Surgery, Heinrich Heine University, Moorenstrasse 5, D-40225, Düsseldorf, Germany aut Ganzer U. Department of Otorhinolaryngology, Head and Neck Surgery, Heinrich Heine University, Moorenstrasse 5, D-40225, Düsseldorf, Germany aut European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/7(1996-09-01), 405-410 0937-4477 253:7<405 1996 253 405 https://doi.org/10.1007/BF00168492 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00168492 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Haas I. Department of Otorhinolaryngology, Head and Neck Surgery, Heinrich Heine University, Moorenstrasse 5, D-40225, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Koldovsky P. Department of Otorhinolaryngology, Head and Neck Surgery, Heinrich Heine University, Moorenstrasse 5, D-40225, Düsseldorf, Germany aut NATIONALLICENCE 700 1- Ganzer U. Department of Otorhinolaryngology, Head and Neck Surgery, Heinrich Heine University, Moorenstrasse 5, D-40225, Düsseldorf, Germany aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/7(1996-09-01), 405-410 0937-4477 253:7<405 1996 253 405 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer