caa a22 4500 477087086 CHVBK 20180405111506.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF00168488 doi (NATIONALLICENCE)springer-10.1007/BF00168488 Mediation of signal transduction in keratinocytes of human middle ear cholesteatoma by ras protein [Elektronische Daten] [C. Huang, C. Chen, T. Huang, H. Shinoda] Immunolocalization was used to investigate the distribution of ras protein in both congenital and acquired cholesteatoma tissues. A pathological feature of middle ear cholesteatoma included accumulation of keratin debris due to active proliferation and differentiation of keratinocytes. Ras protein localized to the inner plasma membrane and cytoplasm of keratinocytes in all of the epithelial layers in both congenital and acquired cholesteatoma specimens. Keratinocytes in the basal layer were stained more intensely than their differentiated counterparts. Findings also demonstrated that receptors for epidermal growth factor, fibroblast growth factor, and plateletderived growth factor were present in the plasma membrane of keratinocytes. C-jun, a transcription factor was found in the nuclei of keratinocytes of the cholesteatoma epithelium. These findings indicate that ras protein plays a pivotal role in a signal transduction cascade between growth factor receptors in the plasma membrane and the transcription factor, c-jun, in the nuclei of keratinocytes during the growth of cholesteatoma. Springer-Verlag, 1996 Cholesteatoma nationallicence Ras protein nationallicence Signal transduction nationallicence Growth factor receptors nationallicence Transcription factor nationallicence Huang C. Department of Otolaryngology-Head and Neck Surgery, Columbia University, 630 West 168th Street, 10032, New York, NY, USA aut Chen C. Department of Otolaryngology-Head and Neck Surgery, Columbia University, 630 West 168th Street, 10032, New York, NY, USA aut Huang T. Department of Otolaryngology, Chang Gung Medical College, Taipei, Taiwan aut Shinoda H. Department of Otolaryngology-Head and Neck Surgery, Columbia University, 630 West 168th Street, 10032, New York, NY, USA aut European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/7(1996-09-01), 385-389 0937-4477 253:7<385 1996 253 405 https://doi.org/10.1007/BF00168488 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00168488 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang C. Department of Otolaryngology-Head and Neck Surgery, Columbia University, 630 West 168th Street, 10032, New York, NY, USA aut NATIONALLICENCE 700 1- Chen C. Department of Otolaryngology-Head and Neck Surgery, Columbia University, 630 West 168th Street, 10032, New York, NY, USA aut NATIONALLICENCE 700 1- Huang T. Department of Otolaryngology, Chang Gung Medical College, Taipei, Taiwan aut NATIONALLICENCE 700 1- Shinoda H. Department of Otolaryngology-Head and Neck Surgery, Columbia University, 630 West 168th Street, 10032, New York, NY, USA aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/7(1996-09-01), 385-389 0937-4477 253:7<385 1996 253 405 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer