caa a22 4500 477087140 CHVBK 20180405111506.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00615113 doi (NATIONALLICENCE)springer-10.1007/BF00615113 Role of interleukin 6 in epithelial hyperproliferation and bone resorption in middle ear cholesteatomas [Elektronische Daten] [J. Bujía, C. Kim, P. Ostos, . Kastenbauer, L. Hültner] Locally produced pro-inflammatory cytokines are considered to play an important role in the initiation and/or maintenance of inflammatory diseases. In cholesteatomatous lesions there are increased levels of some cytokines and inflammatory mediators like interleukin 1, tumor necrosis factor and colony-stimulating factor, etc. Interleukin 6 (IL-6) can be produced by different cells present in cholesteatoma (e.g. keratinocytes, lymphocytes, fibroblasts and macrophages). Until now, no data have been available on the role of IL-6 in cholesteatoma. In this study we used immunohistochemistry to investigate the presence and distribution of IL-6 in tissue samples from cholesteatoma patients. Levels of the cytokine were quantified in tissue extracts using an enzyme-linked immunosorbent assay. Finally, the presence of biologically active IL-6 was analyzed in the murine cell line 7TD1. Human skin samples obtained from the external ear canal were used as controls. Using the anti-IL-6 antibody in an alkaline phosphatase anti alkaline phosphatase technique, a moderate diffuse staining of the whole epidermis was observed in sections of normal skin. In cryostat sections of cholesteatoma samples, a stronger staining of the whole epithelium was observed. Many of the cells infiltrating the cholesteatoma stroma also showed positive immunostainings. The concentration of IL-6 in relation to the total protein concentration in cholesteatoma (119.33 ± 30) were higher than in human skin (9.16 ± 13). While IL-6 activity was not detected in skin samples, two of the ten cholesteatoma samples studied showed a stimulatory effect when incubated with the cell line 7TD1. The overexpression of IL-6 in middle ear cholesteatoma suggests a participation of this cytokine in some of the clinical features seen: epithelial hyperproliferation and bone resorption. The absence of biological activity in the majority of the cholesteatoma samples points to the presence of natural inhibitors for IL-6. Springer-Verlag, 1996 Interleukin 6 nationallicence Enzyme-linked,immunosorbent assay nationallicence Bioassay nationallicence Cholesteatoma nationallicence Bujía J. Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University, Munich, Germany aut Kim C. Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University, Munich, Germany aut Ostos P. Department of Otolaryngology, Head and Neck Surgery, University of Cordoba, Cordoba, Spain aut Kastenbauer Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University, Munich, Germany aut Hültner L. Institute for Experimental Hematology, GSF Research Center for Environmental and Health, Munich, Germany aut European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/3(1996-01-01), 152-157 0937-4477 253:3<152 1996 253 405 https://doi.org/10.1007/BF00615113 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00615113 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bujía J. Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University, Munich, Germany aut NATIONALLICENCE 700 1- Kim C. Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University, Munich, Germany aut NATIONALLICENCE 700 1- Ostos P. Department of Otolaryngology, Head and Neck Surgery, University of Cordoba, Cordoba, Spain aut NATIONALLICENCE 700 1- Kastenbauer Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University, Munich, Germany aut NATIONALLICENCE 700 1- Hültner L. Institute for Experimental Hematology, GSF Research Center for Environmental and Health, Munich, Germany aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/3(1996-01-01), 152-157 0937-4477 253:3<152 1996 253 405 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer