caa a22 4500 477087256 CHVBK 20180405111507.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00176695 doi (NATIONALLICENCE)springer-10.1007/BF00176695 In vitro effects of high-energy pulsed ultrasound on human squamous cell carcinoma cells [Elektronische Daten] [H. Iro, T. Feigl, J. Zenk, F. Waldfahrer] Human squamous cell carcinoma cells cloned from the hypopharynx (FaDu) and oral cavity (SCC-4) were exposed to high-energy pulsed ultrasound (HEPUS) in vitro to evaluate the effects of various physical parameters on cell viability. Such included the number of pulses, voltage applied, pulse repetition rate and cell density. The experimental piezoelectric ultrasound transducer used in the experiments generated pulses with a high negative pressure amplitude. By varying the repetition frequency from 0.6 to 8 Hz, cell viability was found to be least when pulse repetition was approximately 1 Hz. An increase in transducer voltage resulted in a linear decrease in cell viability. The cell survival rate dropped exponentially as a function of the number of pulses applied, reaching 4.2% after 2000 pulses. The cell survival rate exhibited no significant dependence on cell density when cells ranged from 1 to 3.5 · 106 cells ml−1. Data obtained with trypan blue dye exclusion were confirmed by measurements of intracellular lactate dehydrogenase released into an extracellular fluid supernatant. By applying HEPUS to tumor cells, almost complete destruction of the cells could be achieved in vitro. The degree of cell destruction achieved depended significantly on the number of pulses administered, the pulse repetition rate and the transducer voltage applied. Springer-Verlag, 1996 High-energy pulsed ultrasound nationallicence Cell viability nationallicence Squamous cell carcinoma cells nationallicence Treatment nationallicence Iro H. Klinik und Poliklinik für Hals-Nasen-Ohrenkranke, Friedrich-Alexander-Universität Erlangen-Nürnberg, Waldstrasse 1, D-91054, Erlangen, Germany aut Feigl T. Klinik und Poliklinik für Hals-Nasen-Ohrenkranke, Friedrich-Alexander-Universität Erlangen-Nürnberg, Waldstrasse 1, D-91054, Erlangen, Germany aut Zenk J. Klinik und Poliklinik für Hals-Nasen-Ohrenkranke, Friedrich-Alexander-Universität Erlangen-Nürnberg, Waldstrasse 1, D-91054, Erlangen, Germany aut Waldfahrer F. Klinik und Poliklinik für Hals-Nasen-Ohrenkranke, Friedrich-Alexander-Universität Erlangen-Nürnberg, Waldstrasse 1, D-91054, Erlangen, Germany aut European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/1-2(1996-01-01), 11-16 0937-4477 253:1-2<11 1996 253 405 https://doi.org/10.1007/BF00176695 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176695 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iro H. Klinik und Poliklinik für Hals-Nasen-Ohrenkranke, Friedrich-Alexander-Universität Erlangen-Nürnberg, Waldstrasse 1, D-91054, Erlangen, Germany aut NATIONALLICENCE 700 1- Feigl T. Klinik und Poliklinik für Hals-Nasen-Ohrenkranke, Friedrich-Alexander-Universität Erlangen-Nürnberg, Waldstrasse 1, D-91054, Erlangen, Germany aut NATIONALLICENCE 700 1- Zenk J. Klinik und Poliklinik für Hals-Nasen-Ohrenkranke, Friedrich-Alexander-Universität Erlangen-Nürnberg, Waldstrasse 1, D-91054, Erlangen, Germany aut NATIONALLICENCE 700 1- Waldfahrer F. Klinik und Poliklinik für Hals-Nasen-Ohrenkranke, Friedrich-Alexander-Universität Erlangen-Nürnberg, Waldstrasse 1, D-91054, Erlangen, Germany aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/1-2(1996-01-01), 11-16 0937-4477 253:1-2<11 1996 253 405 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer