caa a22 4500 477087329 CHVBK 20180405111507.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00176694 doi (NATIONALLICENCE)springer-10.1007/BF00176694 Preservation of the non-rectangular cuticular plate/cell axis angle of outer hair cells [Elektronische Daten] [H. Kempf, U. Zimmermann, H. Zenner] Motile properties of outer hair cells (OHCs) may contribute to sharp tuning and amplification in the mammalian cochlea. Shape changes of isolated OHCs in response to various physical and chemical influences have been investigated intensively. However, determinations of shape may have been influenced by unanticipated effects of preparation and preservation of the OHCs investigated. Thus, in a first step, lengths of freshly isolated OHCs from the guinea pig cochlea were determined using a video-enhancing magnification system. The cuticular plate/cell axis angle (CP/CA angle) was then measured in native cells and under the influence of potassium chloride and potassium gluconate incubation. To show the influence of glutaraldehyde (GA) fixation on the isolated OHCs, fixative dependent changes on cell length and CP/CA angle were recorded in native and preincubated OHCs. In these experiments, the cell length of vital isolated OHCs was between 41.5 μm, in the basal turn, and 103.7 μm, in the apical turn. The average CP/CA angle was 106° ± 4.2° (n = 324 cells, turns 1-4) with no statistically significant differences for the four turns. Under the influence of potassium chloride, cell length was reduced by 8.1%. Potassium gluconate incubation led to a shortening of cell length, followed by a 5.3% increase after 5 min. The CP/CA angle under potassium chloride was decreased (97.0°) and was then increased under the influence of potassium gluconate (110.7°) as a result of cuticular plate tilting. Cell shrinkage after fixation depended on the fixative's osmolarity and on the GA concentration. Increased GA levels amplified cell shrinkage from 34% for hypo-osmolar solutions to 15% in iso-osmolar and 29% in hyperosmolar solutions. The CP/CA angle of native and incubated OHCs was not different from those fixed with GA. The present data provide a rational basis for isolated OHC shape parameters. Moreover, functionally induced changes can be better interpreted when OHCs are influenced by fixatives, as shown in the GA experiments. Springer-Verlag, 1996 Isolated outer hair cells nationallicence Glutaraldehyde cell fixation nationallicence Cuticular plate nationallicence Cell motility nationallicence Guinea pig nationallicence Kempf H. Medizinische Hochschule Hannover, Department of Otolaryngology, Konstanty-Gutschow-Strasse 8, D-30625, Hannover, Germany aut Zimmermann U. Department of Otolaryngology, Hearing Research Laboratories, University of Tiibingen, Tübingen, Germany aut Zenner H. Department of Otolaryngology, Hearing Research Laboratories, University of Tiibingen, Tübingen, Germany aut European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/1-2(1996-01-01), 5-10 0937-4477 253:1-2<5 1996 253 405 https://doi.org/10.1007/BF00176694 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176694 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kempf H. Medizinische Hochschule Hannover, Department of Otolaryngology, Konstanty-Gutschow-Strasse 8, D-30625, Hannover, Germany aut NATIONALLICENCE 700 1- Zimmermann U. Department of Otolaryngology, Hearing Research Laboratories, University of Tiibingen, Tübingen, Germany aut NATIONALLICENCE 700 1- Zenner H. Department of Otolaryngology, Hearing Research Laboratories, University of Tiibingen, Tübingen, Germany aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/1-2(1996-01-01), 5-10 0937-4477 253:1-2<5 1996 253 405 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer