caa a22 4500 477087345 CHVBK 20180405111507.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF00176697 doi (NATIONALLICENCE)springer-10.1007/BF00176697 Immunohistochemical detection of proliferating cell nuclear antigen in middle ear cholesteatoma [Elektronische Daten] [J. Bujía, H. Sudhoff, A. Holly, H. Hildmann, E. Kastenbauer] Cholesteatoma epithelium is characterized by a keratinocyte dysregulation accompanied by destruction of the ossicles and temporal bone. Immunohistochemical methods using antibodies to cell-cycle-related antigens can be used as a means for assessing various aspects of proliferation in cholesteatoma tissue. They also have the important advantage of preserving the spatial orientation of proliferating cells in histological sections. Proliferating cell nuclear antigen (PCNA) is a 36 kDa DNA-delta-polymerase-associated protein that is directly involved in the mechanisms of DNA synthesis. In the present study the expression of PCNA was investigated in formalin-fixed, paraffin-embedded biopsy specimens of cholesteatomas and normal skin. Normal skin revealed nuclear staining in a small number of keratinocytes (PCNA grade, 1.5) located in the basal cell layer. In contrast, an increased number of PCNA-labeled basal and suprabasal epidermal cells (PCNA grade, 9.3) were found in cholesteatoma samples. Our findings indicate that PCNA represents a reliable marker for epithelial proliferation, showing that cholesteatoma epithelium proliferates at a higher rate than normal epidermis. These findings also support the concept of keratinocyte dysregulation in middle ear cholesteatoma. Springer-Verlag, 1996 Cholesteatoma proliferation nationallicence DNA synthesis nationallicence Proliferating cell nuclear antigen nationallicence Immunohistochemistry nationallicence Bujía J. Department of Otorhinolaryngology, Ludwig-Maximilians-University, Klinikum Großhadern, Marchioninistrasse 15, D-81377, Munich, Germany aut Sudhoff H. Department of Otorhinolaryngology, University of Bochum, Bochum, Germany aut Holly A. Department of Otorhinolaryngology, Ludwig-Maximilians-University, Klinikum Großhadern, Marchioninistrasse 15, D-81377, Munich, Germany aut Hildmann H. Department of Otorhinolaryngology, University of Bochum, Bochum, Germany aut Kastenbauer E. Department of Otorhinolaryngology, Ludwig-Maximilians-University, Klinikum Großhadern, Marchioninistrasse 15, D-81377, Munich, Germany aut European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/1-2(1996-01-01), 21-24 0937-4477 253:1-2<21 1996 253 405 https://doi.org/10.1007/BF00176697 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00176697 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bujía J. Department of Otorhinolaryngology, Ludwig-Maximilians-University, Klinikum Großhadern, Marchioninistrasse 15, D-81377, Munich, Germany aut NATIONALLICENCE 700 1- Sudhoff H. Department of Otorhinolaryngology, University of Bochum, Bochum, Germany aut NATIONALLICENCE 700 1- Holly A. Department of Otorhinolaryngology, Ludwig-Maximilians-University, Klinikum Großhadern, Marchioninistrasse 15, D-81377, Munich, Germany aut NATIONALLICENCE 700 1- Hildmann H. Department of Otorhinolaryngology, University of Bochum, Bochum, Germany aut NATIONALLICENCE 700 1- Kastenbauer E. Department of Otorhinolaryngology, Ludwig-Maximilians-University, Klinikum Großhadern, Marchioninistrasse 15, D-81377, Munich, Germany aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/1-2(1996-01-01), 21-24 0937-4477 253:1-2<21 1996 253 405 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer