caa a22 4500 477087507 CHVBK 20180405111507.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF00179947 doi (NATIONALLICENCE)springer-10.1007/BF00179947 Middle ear catalase distribution in an animal model of otitis media [Elektronische Daten] [R. Parks, C. Huang, J. Haddad Jr.] Increasing evidence implicates free radicals in the pathogenesis of inflammatory disease, including otitis media. The anti-oxidant enzymes catalase, glutathione peroxidase and superoxide dismutase protect tissues from the destructive effects of free radicals. Our previous work has shown depressed levels of superoxide dismutase in the infected middle ears of a guinea pig model of otitis media in comparison with normal control ears. We studied the distribution and relative abundance of catalase in the middle ear of this animal model in an effort to elucidate the role free radicals play in the pathogenesis of otitis media. Catalase distribution was mapped immunohistochemically in the middle ears of guinea pigs with induced streptococcus otitis media, and compared with normal control ears. In the control ears, catalase was localized to the epithelium of the middle ear mucosa, with scant distribution in the submucosa. The infected ears demonstrated inflammatory cell invasion with hyperemia and submucosal edema. Catalase was localized to the epithelium and had scant distribution in the submucosa. This distribution was similar to that found previously with superoxide dismutase. Enzyme-linked immunosorbent assay of catalase demonstrated a mean value of 1.00 ± 0.06 μg/mg protein in the control ears, and 1.06 ± 0.12 μg/mg in the infected ears, but these two values were not statistically different. Springer-Verlag, 1996 Otitis media nationallicence Immunohistochemistry Free radicals nationallicence Catalase nationallicence Superoxide dismutase nationallicence Parks R. Department of Otolaryngology, Division of Pediatric Otolaryngology, Columbia Presbyterian Medical Center, Babies Hospital North-108, 3959 Broadway, 10032, New York, NY, USA aut Huang C. Department of Otolaryngology, Division of Pediatric Otolaryngology, Columbia Presbyterian Medical Center, Babies Hospital North-108, 3959 Broadway, 10032, New York, NY, USA aut Haddad Jr. J. Department of Otolaryngology, Division of Pediatric Otolaryngology, Columbia Presbyterian Medical Center, Babies Hospital North-108, 3959 Broadway, 10032, New York, NY, USA aut European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/8(1996-11-01), 445-449 0937-4477 253:8<445 1996 253 405 https://doi.org/10.1007/BF00179947 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00179947 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Parks R. Department of Otolaryngology, Division of Pediatric Otolaryngology, Columbia Presbyterian Medical Center, Babies Hospital North-108, 3959 Broadway, 10032, New York, NY, USA aut NATIONALLICENCE 700 1- Huang C. Department of Otolaryngology, Division of Pediatric Otolaryngology, Columbia Presbyterian Medical Center, Babies Hospital North-108, 3959 Broadway, 10032, New York, NY, USA aut NATIONALLICENCE 700 1- Haddad Jr J. Department of Otolaryngology, Division of Pediatric Otolaryngology, Columbia Presbyterian Medical Center, Babies Hospital North-108, 3959 Broadway, 10032, New York, NY, USA aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/8(1996-11-01), 445-449 0937-4477 253:8<445 1996 253 405 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer