caa a22 4500 477087531 CHVBK 20180405111507.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF00179950 doi (NATIONALLICENCE)springer-10.1007/BF00179950 Experimental erbium laser surgery in the guinea pig cochlea: its use in the study of afferent cochlear neurotransmitters [Elektronische Daten] [A. Arnold, D. Felix, K. Ehrenberger, M. Frenz, H. Pratisto, H. Weber, H. Altermatt] Microiontophoretic techniques were used to examine the changes in activity of inner hair cell afferents in the guinea pig following circumscribed penetration of cochlear bone with erbium:YSGG laser pulses. Neuronal responses to the application of the glutamate agonists N-methyl-d-aspartate (NMDA) and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) before laser use and after return to normal spontaneous activity were equivalent, implying total reversibility of the changes occurring. Suppression of laser-induced hair cell activity was possible with NMDA and AMPA receptor antagonists and lasted 10-15 min. These findings suggest a transmitter-related increase of neuronal activity. Our results show that use of the erbium laser in inner ear microsurgery might be possible with low risk if the amount of energy applied is kept under a safe limit of 10 J/cm2. Springer-Verlag, 1996 Inner hair cells nationallicence Cochlear afferent transmitters nationallicence Glutamate agonists nationallicence Erbium laser surgery Iontophoresis nationallicence Arnold A. Division of Neurobiology, University of Berne, Erlachstrasse 9a, CH-3012, Berne, Switzerland aut Felix D. Division of Neurobiology, University of Berne, Erlachstrasse 9a, CH-3012, Berne, Switzerland aut Ehrenberger K. ENT Department, University of Vienna, Währinger Gürtel 18-20, A-1090, Vienna, Austria aut Frenz M. Institute of Applied Physics, University of Berne, Sidlerstrasse 5, CH-3012, Berne, Switzerland aut Pratisto H. Institute of Applied Physics, University of Berne, Sidlerstrasse 5, CH-3012, Berne, Switzerland aut Weber H. Institute of Applied Physics, University of Berne, Sidlerstrasse 5, CH-3012, Berne, Switzerland aut Altermatt H. Institute of Pathology, University of Berne, Murtenstrasse 31, CH-3010, Berne, Switzerland aut European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/8(1996-11-01), 460-463 0937-4477 253:8<460 1996 253 405 https://doi.org/10.1007/BF00179950 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00179950 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arnold A. Division of Neurobiology, University of Berne, Erlachstrasse 9a, CH-3012, Berne, Switzerland aut NATIONALLICENCE 700 1- Felix D. Division of Neurobiology, University of Berne, Erlachstrasse 9a, CH-3012, Berne, Switzerland aut NATIONALLICENCE 700 1- Ehrenberger K. ENT Department, University of Vienna, Währinger Gürtel 18-20, A-1090, Vienna, Austria aut NATIONALLICENCE 700 1- Frenz M. Institute of Applied Physics, University of Berne, Sidlerstrasse 5, CH-3012, Berne, Switzerland aut NATIONALLICENCE 700 1- Pratisto H. Institute of Applied Physics, University of Berne, Sidlerstrasse 5, CH-3012, Berne, Switzerland aut NATIONALLICENCE 700 1- Weber H. Institute of Applied Physics, University of Berne, Sidlerstrasse 5, CH-3012, Berne, Switzerland aut NATIONALLICENCE 700 1- Altermatt H. Institute of Pathology, University of Berne, Murtenstrasse 31, CH-3010, Berne, Switzerland aut NATIONALLICENCE 773 0- European Archives of Oto-Rhino-Laryngology Springer-Verlag 253/8(1996-11-01), 460-463 0937-4477 253:8<460 1996 253 405 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer