caa a22 4500 477089232 CHVBK 20180405111511.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF02254940 doi (NATIONALLICENCE)springer-10.1007/BF02254940 Preparation of tomato meiotic pachytene and mitotic metaphase chromosomes suitable for fluorescence in situ hybridization (FISH) [Elektronische Daten] [Xiao-Bo Zhong, J. de Jong, Pim Zabel] Fluorescencein situ hybridization (FISH) is an increasingly powerful tool with a variety of applications in both basic and applied research. With excellent genetic, cytogenetic and molecular maps available, the tomato genome provides a good model to benefit from the full potential of FISH. Tomato chromosomes at mitotic metaphase are small and not particularly suitable for high-resolution FISH. In contrast, chromosomes at meiotic pachytene are about 15 times longer, and easier to identify by their differences in chromosome arm lengths and chromomere pattern. We have developed a technique for preparing chromosomal spreads of young pollen mother cells at midprophase I which is suitable for FISH. In a first series of experiments, the hybridization patterns of three classes of repetitive DNA sequences were studied in single and multicolour FISH. Rapid Science Publishers, 1996 fluorescence in situ hybridization nationallicence pachytene nationallicence metaphase nationallicence repetitive sequences nationallicence tomato nationallicence Zhong Xiao-Bo Department of Molecular Biology, Wageningen Agricultural University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands aut de Jong J. Department of Genetics, Wageningen Agricultural University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands aut Zabel Pim Department of Molecular Biology, Wageningen Agricultural University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands aut Chromosome Research Kluwer Academic Publishers 4/1(1996-01-01), 24-28 0967-3849 4:1<24 1996 4 10577 https://doi.org/10.1007/BF02254940 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02254940 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhong Xiao-Bo Department of Molecular Biology, Wageningen Agricultural University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- de Jong J. Department of Genetics, Wageningen Agricultural University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Zabel Pim Department of Molecular Biology, Wageningen Agricultural University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/1(1996-01-01), 24-28 0967-3849 4:1<24 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer