caa a22 4500 477089240 CHVBK 20180405111511.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF02254945 doi (NATIONALLICENCE)springer-10.1007/BF02254945 The chromosomal distribution and organization of sheep satellite I and II centromeric DNA using characterized sheep-hamster somatic cell hybrids [Elektronische Daten] [Dean Burkin, Thomas Broad, Carol Jones] A panel of sheep-hamster somatic cell hybrids containing single sheep chromosomes was used to study the chromosomal distribution and organization of two families of sheep centromeric satellite DNA. This study shows that the centromeres of the sheep metacentric chromosomes 1,2 and 3 differ in their organization and relative quantities of sheep satellite I DNA. The results, when correlated with the proposed formation of these metacentric chromosomes by ancient Robertsonian translocations, suggest a loss or replacement of satellite I centromeric DNA from the centromeres of these sheep chromosomes. Using Southern blot analysis and fluorescencein situ hybridization, this study shows that the recent centric fusion chromosome t2 (rob 9;10) contains little satellite II DNA. Together these resuults suggest the possibility of substantial reorganization of sheep centromeric DNA families after Robertsonian translocations. Rapid Science Publishers, 1996 centromere nationallicence Ovis aries nationallicence Robertsonian translocations nationallicence satellite DNA nationallicence somatic cell hybrids nationallicence Burkin Dean Eleanor Roosevelt Institute, 1899 Gaylord Street, 80206, Denver, CO, USA aut Broad Thomas AgResearch, Invermay Agricultural Center, Private Bag 50034, Mosgiel, New Zealand aut Jones Carol Eleanor Roosevelt Institute, 1899 Gaylord Street, 80206, Denver, CO, USA aut Chromosome Research Kluwer Academic Publishers 4/1(1996-01-01), 49-55 0967-3849 4:1<49 1996 4 10577 https://doi.org/10.1007/BF02254945 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02254945 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Burkin Dean Eleanor Roosevelt Institute, 1899 Gaylord Street, 80206, Denver, CO, USA aut NATIONALLICENCE 700 1- Broad Thomas AgResearch, Invermay Agricultural Center, Private Bag 50034, Mosgiel, New Zealand aut NATIONALLICENCE 700 1- Jones Carol Eleanor Roosevelt Institute, 1899 Gaylord Street, 80206, Denver, CO, USA aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/1(1996-01-01), 49-55 0967-3849 4:1<49 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer