caa a22 4500 477089259 CHVBK 20180405111511.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF02254947 doi (NATIONALLICENCE)springer-10.1007/BF02254947 Tease Charles MRC Radiobiology Unit, Chilton, OX11 ORD, Didcot, Oxon, UK aut Analysis using dual-colour fluorescence in situ hybridization of meiotic chromosome segregation in male mice heterozygous for a reciprocal translocation [Elektronische Daten] [Charles Tease] Meiotic chromosome behaviour was investigated in male mice heterozygous for the translocation T(7;16)67H. At metaphase I, chain-of-four quadrivalents were present in approximately 80% of the spermatocytes; the bulk of remaining cells contained a ring quadrivalent, with only a few having either a trivalent plus univalent configuration or two bivalents. A low rate of non-disjunction, approximately 5%, was found through analysis of C-banded metaphase II spermatocytes. Using fluorescencein situ hybridization with differentially labelled whole chromosome paints, a wide array of segregation products were observed at metaphase II, depending on whether they arose from alternate, adjacent I, adjacent II orientation at metaphase I or were uninformative for alternative/adjacent I because of the presence of a chiasma in an interstitial pairing segment. Some 62% of the cells fell into this latter category, with only small proportions clearly arising through alternate (1.8%) or adjacent I (0.7%) orientations. Approximately 30% of the cells contained the products of adjacent II orientation. Consideration of the data suggested that most of these cells arose from metaphase I cells that contained a chain quadrivalent. Ring quadrivalents appeared predominantly to orientate in an alternate/adjacent I manner. Rapid Science Publishers, 1996 chromosome painting nationallicence fluorescence in situ hybridization nationallicence meiosis nationallicence mouse nationallicence reciprocal translocation nationallicence Chromosome Research Kluwer Academic Publishers 4/1(1996-01-01), 61-68 0967-3849 4:1<61 1996 4 10577 https://doi.org/10.1007/BF02254947 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02254947 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Tease Charles MRC Radiobiology Unit, Chilton, OX11 ORD, Didcot, Oxon, UK aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/1(1996-01-01), 61-68 0967-3849 4:1<61 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer