caa a22 4500 477089275 CHVBK 20180405111512.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF02254939 doi (NATIONALLICENCE)springer-10.1007/BF02254939 Molecular analysis of the structure of the maize B-chromosome [Elektronische Daten] [Elizabeth Stark, Ian Connerton, Simon Bennett, Stephen Barnes, John Parker, John Forster] The overall composition of the maize B is similar to that of the standard chromosome complement (A-chromosomes). This has been demonstrated by the use of several methods including: (a) genomicin situ hybridization (GISH), (b) analysis of highly repetitive sequences by the comparison of restriction digests of 0B and +B DNA and (c) the characterization of middle-repetitive cloned sequences. By the use of the technique of random amplification of polymorphic DNA-polymerase chain reaction, we have identified a B-specific repetitive sequence family. Sequence analysis of the B-specific clone reveals a relationship to the PREM-1 family of maize retroelements, which are preferentially transcribed in pollen. These results suggest an internal origin of the B-chromosome from within the maize genome, but demonstrate also that specific sequences can evolve by rapid processes of genomic turnover. Models for the origin of the maize B are discussed in the context of the present data. Rapid Science Publishers, 1996 B-chromosome nationallicence DNA sequence composition nationallicence PREM-1 element nationallicence random amplification of polymorphic DNA nationallicence Zea mays nationallicence Stark Elizabeth Department of Botany, Plant Science Laboratories, University of Reading, Whiteknights, RG6 2AS, Reading, UK aut Connerton Ian Institute of Food Research, Reading Laboratory, Earley Gate, Whiteknight Road, RG6 2EF, Reading, UK aut Bennett Simon Nuffield Department of Surgery, University of Oxford, John Radcliffe Hospital, OX3 9DU, Headington, Oxford, UK aut Barnes Stephen Zeneca Seeds CES/MV/SA, Industriepark 15, B-3300, Tienen, Belgium aut Parker John Department of Botany, Plant Science Laboratories, University of Reading, Whiteknights, RG6 2AS, Reading, UK aut Forster John Genetics Group, Institute of Biological Sciences, UW Aberystwyth, SY23 3DD, Penglais, Aberystwyth, UK aut Chromosome Research Kluwer Academic Publishers 4/1(1996-01-01), 15-23 0967-3849 4:1<15 1996 4 10577 https://doi.org/10.1007/BF02254939 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02254939 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stark Elizabeth Department of Botany, Plant Science Laboratories, University of Reading, Whiteknights, RG6 2AS, Reading, UK aut NATIONALLICENCE 700 1- Connerton Ian Institute of Food Research, Reading Laboratory, Earley Gate, Whiteknight Road, RG6 2EF, Reading, UK aut NATIONALLICENCE 700 1- Bennett Simon Nuffield Department of Surgery, University of Oxford, John Radcliffe Hospital, OX3 9DU, Headington, Oxford, UK aut NATIONALLICENCE 700 1- Barnes Stephen Zeneca Seeds CES/MV/SA, Industriepark 15, B-3300, Tienen, Belgium aut NATIONALLICENCE 700 1- Parker John Department of Botany, Plant Science Laboratories, University of Reading, Whiteknights, RG6 2AS, Reading, UK aut NATIONALLICENCE 700 1- Forster John Genetics Group, Institute of Biological Sciences, UW Aberystwyth, SY23 3DD, Penglais, Aberystwyth, UK aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/1(1996-01-01), 15-23 0967-3849 4:1<15 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer