caa a22 4500 477089313 CHVBK 20180405111512.0 cr unu---uuuuu 170330e19960101xx s 000 0 eng 10.1007/BF02254942 doi (NATIONALLICENCE)springer-10.1007/BF02254942 Mapping of 22 Notl linking clones on human chromosome 3 by polymerase chain reaction and somatic cell hybrid panels [Elektronische Daten] [R. Allikmets, V. Kashuba, K. Huebner, S. LaForgia, L. Kisselev, G. Klein, M. Dean, E. Zabarovsky] Twenty-two human chromosome 3 derived and partially sequencedNotl linking clones were mapped using two somatic cell hybrid panels. Somatic cell hybrid mapping was performed by Southern hybridization and/or by polymerase chain reaction (PCR), using 300-500 bp CpG-rich sequences surroundingNotl sites. Thus, 22 newNotl site-tagged (sequence tagged sites) STSs were created, distributed over the entire human chromosome 3. The majority of these linking clones tag known or unknown expressed sequences (genes). Together with other physical and genetic mapping methods, localization ofNotl linking clones facilitates the construction of a long-range physical map and, at the same time, a transcriptional map of human chromosome 3. Rapid Science Publishers, 1996 chromosome 3 nationallicence Notl linking clones nationallicence sequence tagged sites nationallicence somatic cell hybrids nationallicence Allikmets R. Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick Cancer Research and Development Center, 21702-1201, Frederick, MD, USA aut Kashuba V. Department of Tumor Biology, Karolinska Institutet, Box 60400, S-10401, Stockholm, Sweden aut Huebner K. Jefferson Cancer Institute, Jefferson Medical College, 19103, Philadelphia, PA, USA aut LaForgia S. Jefferson Cancer Institute, Jefferson Medical College, 19103, Philadelphia, PA, USA aut Kisselev L. Engelhardt Institute of Molecular Biology, Vavilov Str. 32, 117984, Moscow, USA aut Klein G. Department of Tumor Biology, Karolinska Institutet, Box 60400, S-10401, Stockholm, Sweden aut Dean M. Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick Cancer Research and Development Center, 21702-1201, Frederick, MD, USA aut Zabarovsky E. Department of Tumor Biology, Karolinska Institutet, Box 60400, S-10401, Stockholm, Sweden aut Chromosome Research Kluwer Academic Publishers 4/1(1996-01-01), 33-37 0967-3849 4:1<33 1996 4 10577 https://doi.org/10.1007/BF02254942 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02254942 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Allikmets R. Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick Cancer Research and Development Center, 21702-1201, Frederick, MD, USA aut NATIONALLICENCE 700 1- Kashuba V. Department of Tumor Biology, Karolinska Institutet, Box 60400, S-10401, Stockholm, Sweden aut NATIONALLICENCE 700 1- Huebner K. Jefferson Cancer Institute, Jefferson Medical College, 19103, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- LaForgia S. Jefferson Cancer Institute, Jefferson Medical College, 19103, Philadelphia, PA, USA aut NATIONALLICENCE 700 1- Kisselev L. Engelhardt Institute of Molecular Biology, Vavilov Str. 32, 117984, Moscow, USA aut NATIONALLICENCE 700 1- Klein G. Department of Tumor Biology, Karolinska Institutet, Box 60400, S-10401, Stockholm, Sweden aut NATIONALLICENCE 700 1- Dean M. Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick Cancer Research and Development Center, 21702-1201, Frederick, MD, USA aut NATIONALLICENCE 700 1- Zabarovsky E. Department of Tumor Biology, Karolinska Institutet, Box 60400, S-10401, Stockholm, Sweden aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/1(1996-01-01), 33-37 0967-3849 4:1<33 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer