caa a22 4500 477089356 CHVBK 20180405111512.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF02265049 doi (NATIONALLICENCE)springer-10.1007/BF02265049 Conservation of a 31-bp bovine subrepeat in centromeric satellite DNA monomers of Cervus elaphus and other cervid species [Elektronische Daten] [C. Lee, C. Lin] A centromeric satellite DNA clone was isolated from the genome of the European red deer (Cervus elaphus hippelaphus) and designated Ce-Pst1. This clone was localized to the centromeric region of all red deer chromosomes with the exception of a single pair of metacentric autosomes and the Y chromosome. DNA sequence analysis of the 806-bp Ce-Pst1 clone showed 73.0-78.9% sequence homology to four previously isolated cervid centromeric satellite DNA clones, suggesting that the Ce-Pst1 clone is yet another member of the major cervid centromeric satellite DNA family. Using a DNA sequence comparison system, internal 31-bp tandem subrepeats were found in the Ce-Pst1 clone as well as in the other previously reported cervid centromeric satellite DNA monomer sequences. A 31-bp consensus sequence was constructed for each cervid monomer clone and shown to be highly homologous to the 31-bp subrepeat consensus sequence found in bovine 1.715 centromeric satellite DNA. The identification of internal subrepeats in the satellite monomers studied could suggest that amplification of an ancestral 31-bp DNA sequence may have contributed to the genesis of major cervid centromeric satellite DNA. The homology between the 31-bp subrepeats found in cervid and bovid centromeric satellite DNAs substantiates the theory that amplification of this 31-bp DNA sequence may have occurred before the evolutionary separation of these two families 20-25 million years ago. Rapid Science Publishers, 1996 centromere nationallicence internal repeats nationallicence repetitive DNA evolution nationallicence satellite DNA nationallicence Lee C. Cytogenetics Laboratory, 5B4.49 W. C. Mackenzie Health Sciences Centre, University of Alberta Hospitals, 8440-112 Street, T6G 2B7, Edmonton, Alberta, Canada aut Lin C. Cytogenetics Laboratory, 5B4.49 W. C. Mackenzie Health Sciences Centre, University of Alberta Hospitals, 8440-112 Street, T6G 2B7, Edmonton, Alberta, Canada aut Chromosome Research Kluwer Academic Publishers 4/6(1996-09-01), 427-435 0967-3849 4:6<427 1996 4 10577 https://doi.org/10.1007/BF02265049 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02265049 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee C. Cytogenetics Laboratory, 5B4.49 W. C. Mackenzie Health Sciences Centre, University of Alberta Hospitals, 8440-112 Street, T6G 2B7, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Lin C. Cytogenetics Laboratory, 5B4.49 W. C. Mackenzie Health Sciences Centre, University of Alberta Hospitals, 8440-112 Street, T6G 2B7, Edmonton, Alberta, Canada aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/6(1996-09-01), 427-435 0967-3849 4:6<427 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer