caa a22 4500 477089445 CHVBK 20180405111512.0 cr unu---uuuuu 170330e19960901xx s 000 0 eng 10.1007/BF02265048 doi (NATIONALLICENCE)springer-10.1007/BF02265048 Characterization of DNA sequences constituting the terminal heterochromatin of the chicken Z chromosome [Elektronische Daten] [Tetsuya Hori, Yukiko Suzuki, Irina Solovei, Yasushi Saitoh, Nancy Hutchison, Joh-E Ikeda, Herbert Macgregor, Shigeki Mizuno] Two clones, pCZTH5-8 and pCZTH12-8, were isolated from a female chicken genomic library by screening with sequences obtained from genomic libraries which had been constructed from a terminal region of a single Z chromosome of chicken utilizing laser microbeam irradiation and PCR amplification. Fluorescencein situ hybridization to the mitotic Z chromosome and the lampbrush ZW bivalent of chicken demonstrated that both the cloned sequences are located in the heterochromatic region of the Z chromosome at the end opposite to the pairing region with the W chromosome. The sequences pCZTH5-8 and pCZTH12-8 are distributed widely on both the telomeric bow-like loops (TBL) and the region I (short loops region) of the Z lampbrush chromosome. These clones, pCZTH12-8 particularly notably, hybridized also to the TBLs of lampbrush bivalents 1-4 of chicken. Both sequence are transcribed in the lampbrush stage oocytes on the Z chromosome and on other macrobivalents. The subfragment of pCZTH5-8 which hybridizes to the TBLs and the insert of pCZTH12-8 contain regions that are closely similar in sequence. The pCZTH5-8 sequence has no internal repeats and may be part of the 24-kb macrosatellite repeating unit that is evident afterNhel digestion of the genomic DNA. A cloned 24-kb unit, pFN-1, does not show significant DNA curvature, but cytosines of its CpG dinucleotides may be highly methylatedin vivo. This contrasts with the repeat sequences of the W heterochromatin which not only have highly methylated CpG but are also strongly curved. The 24-kb unit is repeated about 830 times in the diploid genome of a female chicken, suggesting that nearly the entire terminal heterochromatin on the Z chromosome consists of this macrosatellite family. Sequences of the greater part of the pCZTH5-8 are restricted to the genusGallus but the sequence of one subregion which hybridizes to TBLs is present in the genomes of the order Galliformes. Rapid Science Publishers, 1996 fluorescence in situ hybridization nationallicence Gallus nationallicence heterochromatin nationallicence macrosatellite DNA nationallicence Z chromosome nationallicence Hori Tetsuya Laboratory of Molecular Biology, Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Aoba-ku, 981, Sendai, Japan aut Suzuki Yukiko Laboratory of Molecular Biology, Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Aoba-ku, 981, Sendai, Japan aut Solovei Irina Biological Institute, University of St Petersburg, St Petersburg, Russia aut Saitoh Yasushi Institute of Medical Science, Tokai University School of Medicine, Isehara, Kanagawa, Japan aut Hutchison Nancy Fred Hutchinson Cancer Research Center, Seattle, WA, USA aut Ikeda Joh-E Institute of Medical Science, Tokai University School of Medicine, Isehara, Kanagawa, Japan aut Macgregor Herbert Department of Zoology, University of Leicester, Leicester, UK aut Mizuno Shigeki Laboratory of Molecular Biology, Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Aoba-ku, 981, Sendai, Japan aut Chromosome Research Kluwer Academic Publishers 4/6(1996-09-01), 411-426 0967-3849 4:6<411 1996 4 10577 https://doi.org/10.1007/BF02265048 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02265048 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hori Tetsuya Laboratory of Molecular Biology, Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Aoba-ku, 981, Sendai, Japan aut NATIONALLICENCE 700 1- Suzuki Yukiko Laboratory of Molecular Biology, Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Aoba-ku, 981, Sendai, Japan aut NATIONALLICENCE 700 1- Solovei Irina Biological Institute, University of St Petersburg, St Petersburg, Russia aut NATIONALLICENCE 700 1- Saitoh Yasushi Institute of Medical Science, Tokai University School of Medicine, Isehara, Kanagawa, Japan aut NATIONALLICENCE 700 1- Hutchison Nancy Fred Hutchinson Cancer Research Center, Seattle, WA, USA aut NATIONALLICENCE 700 1- Ikeda Joh-E Institute of Medical Science, Tokai University School of Medicine, Isehara, Kanagawa, Japan aut NATIONALLICENCE 700 1- Macgregor Herbert Department of Zoology, University of Leicester, Leicester, UK aut NATIONALLICENCE 700 1- Mizuno Shigeki Laboratory of Molecular Biology, Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Aoba-ku, 981, Sendai, Japan aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/6(1996-09-01), 411-426 0967-3849 4:6<411 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer