caa a22 4500 477089496 CHVBK 20180405111512.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF02261778 doi (NATIONALLICENCE)springer-10.1007/BF02261778 A light microscopic atlas of meiosis in Arabidopsis thaliana [Elektronische Daten] [K. Ross, P. Fransz, G. Jones] An atlas of meiosis inArabidopsis thaliana, encompassing all stages from preleptotene to telophase II and early microspore formation, is presented in detail for the first time. The photomicrographs and descriptions are based on staining with the DNA fluorochrome 4′,6-diamidino-2-phenylindole (DAPI) combined with a spreading procedure, or haematoxylin-iron alum (HIA) staining. Despite previous reservations about the practicality of cytogenetic meiotic analysis inArabidopsis due to its small genome size, good-quality and clearly analysable preparations of all meiotic stages were obtained. This atlas of normal, wild-type meiosis is considered an essential prerequisite to informed analyses of meiotic mutants. Furthermore, extended prophase I chromosomes, particularly at the pachytene stage, offer considerable potential for producing a detailed cytogenetic map (karyotype) ofArabidopsis chromosomes with the additional prospect of high-resolution physical mapping based on fluorescencein situ hybridization (FISH) of defined DNA probes to these extended chromosomes. Rapid Science Publishers, 1996 Arbidopsis nationallicence cytogenetics nationallicence light microscopy nationallicence meiosis nationallicence chromosomes nationallicence Ross K. School of Biological Sciences, The University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK aut Fransz P. School of Biological Sciences, The University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK aut Jones G. School of Biological Sciences, The University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK aut Chromosome Research Kluwer Academic Publishers 4/7(1996-11-01), 507-516 0967-3849 4:7<507 1996 4 10577 https://doi.org/10.1007/BF02261778 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02261778 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ross K. School of Biological Sciences, The University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK aut NATIONALLICENCE 700 1- Fransz P. School of Biological Sciences, The University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK aut NATIONALLICENCE 700 1- Jones G. School of Biological Sciences, The University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/7(1996-11-01), 507-516 0967-3849 4:7<507 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer