caa a22 4500 477089518 CHVBK 20180405111512.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF02261781 doi (NATIONALLICENCE)springer-10.1007/BF02261781 Construction of chromosome-specific DNA libraries covering the whole genome of field bean ( Vicia faba L.) [Elektronische Daten] [J. Macas, G. Gualberti, M. Nouzová, P. Samec, S. Lucretti, J. Doležel] Recombinant DNA libraries were constructed for seven chromosome types isolated from two translocation lines of field bean (Vicia faba L.) with reconstructed karyotypes. The chromosomes were selected so that the set of libraries covers the wholeV. faba genome more than once. Individual chromosome types were highly purified by flow sorting, and their DNA was amplified by degenerate oligonucleotideprimed (DOP) polymerase chain reaction (PCR) and cloned into a plasmid vector. The choice of restriction site present in PCR primer and refinement of cloning protocol resulted in high cloning efficiency and allowed generation of libraries consisting of about 106 clones from 250 or 1000 sorted chromosomes. The insert size ranged between 50 and 2200 bp and the mean length estimated in individual libraries varied between 310 and 487 pb. Hybridization of cloned fragments with labelled genomic DNA showed that about 60% of inserts represented unique or low-copy sequences. The suitability of the libraries for genome mapping was demonstrated by isolation of clones containing microsatellite motifs. Rapid Science Publishers, 1996 chromosome flow sorting nationallicence degenerate oligonucleotide-primed polymerase chain reaction nationallicence chromosome-specific DNA libraries nationallicence genome mapping nationallicence Vicia faba nationallicence Macas J. Institute of Plant Molecular Biology, Branišovská 31, CZ-370 05, České Budějovice, Czech Republic aut Gualberti G. Institute of Plant Molecular Biology, Branišovská 31, CZ-370 05, České Budějovice, Czech Republic aut Nouzová M. Institute of Plant Molecular Biology, Branišovská 31, CZ-370 05, České Budějovice, Czech Republic aut Samec P. Institute of Plant Molecular Biology, Branišovská 31, CZ-370 05, České Budějovice, Czech Republic aut Lucretti S. Biotechnology and Agriculture Division, ENEA, CR Casaccia, Via Anguillarese 301, 00060 SM di Galeria, Rome, Italy aut Doležel J. Institute of Experimental Botany, De Montfort University Norman Borlaug Centre for Plant Science Research, Sokolovská 6, CZ-772 00, Olomouc, Czech Republic aut Chromosome Research Kluwer Academic Publishers 4/7(1996-11-01), 531-539 0967-3849 4:7<531 1996 4 10577 https://doi.org/10.1007/BF02261781 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02261781 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Macas J. Institute of Plant Molecular Biology, Branišovská 31, CZ-370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 700 1- Gualberti G. Institute of Plant Molecular Biology, Branišovská 31, CZ-370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 700 1- Nouzová M. Institute of Plant Molecular Biology, Branišovská 31, CZ-370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 700 1- Samec P. Institute of Plant Molecular Biology, Branišovská 31, CZ-370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 700 1- Lucretti S. Biotechnology and Agriculture Division, ENEA, CR Casaccia, Via Anguillarese 301, 00060 SM di Galeria, Rome, Italy aut NATIONALLICENCE 700 1- Doležel J. Institute of Experimental Botany, De Montfort University Norman Borlaug Centre for Plant Science Research, Sokolovská 6, CZ-772 00, Olomouc, Czech Republic aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/7(1996-11-01), 531-539 0967-3849 4:7<531 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer