caa a22 4500 477089550 CHVBK 20180405111512.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF02261782 doi (NATIONALLICENCE)springer-10.1007/BF02261782 On the importance of DNA strand breaks as the first event to initiate sister chromatid exchange (SCE): Experiments with restriction endonuclease Bgl I [Elektronische Daten] [T. Ortiz, J. Piñero, F. Cortés] The exact molecular mechanism of sister chromatid exchange (SCE) is still unknown, despite the many reports dealing with this cytogenetic end point published in the last 40 years. One point to be investigated is the nature of the original lesion(s) in DNA leading to the production of SCE. Whereas, for chromosomal aberrations, the importance of DNA double-strand breaks has been well established, there is still controversy about the relative importance of strand breaks and base modifications for triggering the process of SCE formation. In the present paper, we have taken advantage of the ability of the restriction endonucleaseBglI to induce SCE and have exploited the fact that preincubation with 2,3-butanedione results in the loss ofBglI ability to cut DNA, while it is still able to recognize its sequence in DNA and bind to it, to see whether this alone is enough to initiate SCE formation, or if a physical DNA double-strand break is required. Our results seem to support the necessity of DNA breaks for SCE production. Rapid Science Publishers, 1996 chromosome aberration nationallicence DNA damage nationallicence restriction endonuclease nationallicence sister chromatid exchange nationallicence Ortiz T. Department of Cell Biology, Faculty of Biology, Avenida Reina Mercedes s/n, E-41012, Seville, Spain aut Piñero J. Department of Cell Biology, Faculty of Biology, Avenida Reina Mercedes s/n, E-41012, Seville, Spain aut Cortés F. Department of Cell Biology, Faculty of Biology, Avenida Reina Mercedes s/n, E-41012, Seville, Spain aut Chromosome Research Kluwer Academic Publishers 4/7(1996-11-01), 540-544 0967-3849 4:7<540 1996 4 10577 https://doi.org/10.1007/BF02261782 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02261782 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ortiz T. Department of Cell Biology, Faculty of Biology, Avenida Reina Mercedes s/n, E-41012, Seville, Spain aut NATIONALLICENCE 700 1- Piñero J. Department of Cell Biology, Faculty of Biology, Avenida Reina Mercedes s/n, E-41012, Seville, Spain aut NATIONALLICENCE 700 1- Cortés F. Department of Cell Biology, Faculty of Biology, Avenida Reina Mercedes s/n, E-41012, Seville, Spain aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/7(1996-11-01), 540-544 0967-3849 4:7<540 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer