caa a22 4500 477089585 CHVBK 20180405111512.0 cr unu---uuuuu 170330e19961101xx s 000 0 eng 10.1007/BF02261776 doi (NATIONALLICENCE)springer-10.1007/BF02261776 Physical mapping of repetitive DNA sequences and 5S and 18S-26S rDNA in five wild species of the genus Hordeum [Elektronische Daten] [A. de Bustos, A. Cuadrado, C. Soler, N. Jouve] The genetic relationships between several wild species and subspecies of the genusHordeum were assessed using fluorescencein situ hybridization (FISH). Plant material included natural populations of wild barley growing in Spain of the annual species,H. marinum ssp.marinum (2n=14) andgussoneanum (2n=14), andH. murinum ssp.murinum (2n=28), andleporinum (2n=28) and the perennial speciesH. bulbosum (2n=14) andH. secalinum (2n=28), plus the South American perennial speciesH. chilense (2n=14). FISH was used to locate the chromosomal sites of two rDNA multigene families 5S and 18S-26S (pTa71 and pTa794) and three repetitive DNA sequences (pSc119.2, pAs1 and pHch950) isolated from different species and genera. The seven chromosomes of the diploid species were readily distinguished by their external morphology and hybridization patterns to pTa71, pTa794, pSc119.2 and pAs1. These DNA probes were also useful for the identification of homologous chromosomes and in differentiating these from unidentified chromosomes in the tetraploid taxa. The use of the probe pHch950 permitted intergenomic differentiation in tetraploids and supports the diphyletic origin ofH. murinum andH. secalinum. Thein situ experiments yielded the following conclusions: (1) differences between the subspeciesmarinum andgussoneanum; (2) close relationships between the subspeciesmurinum andLeporinum; and (3) major differences in physical mapping betweenH. bulbosum and the remaining taxa. The genomic and phylogenetic relationships between taxa, as inferred from the results, are discussed. Rapid Science Publishers, 1996 barley, fluorescence in situ hybridization nationallicence Hordeum bulbosum nationallicence Hordeum chilense nationallicence Hordeum marinum nationallicence Hordeum murinum nationallicence Hordeum secalinum nationallicence repetitive DNA nationallicence de Bustos A. Department of Plant Breeding, CIT, INIA, La Canaleja, PO Box 1100, 28800, Alcalá de Henares (Madrid), Spain aut Cuadrado A. Department of Cell Biology and Genetics, University of Alcalá de Henares, 28871, Alcalá de Henares (Madrid), Spain aut Soler C. Department of Plant Breeding, CIT, INIA, La Canaleja, PO Box 1100, 28800, Alcalá de Henares (Madrid), Spain aut Jouve N. Department of Cell Biology and Genetics, University of Alcalá de Henares, 28871, Alcalá de Henares (Madrid), Spain aut Chromosome Research Kluwer Academic Publishers 4/7(1996-11-01), 491-499 0967-3849 4:7<491 1996 4 10577 https://doi.org/10.1007/BF02261776 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02261776 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Bustos A. Department of Plant Breeding, CIT, INIA, La Canaleja, PO Box 1100, 28800, Alcalá de Henares (Madrid), Spain aut NATIONALLICENCE 700 1- Cuadrado A. Department of Cell Biology and Genetics, University of Alcalá de Henares, 28871, Alcalá de Henares (Madrid), Spain aut NATIONALLICENCE 700 1- Soler C. Department of Plant Breeding, CIT, INIA, La Canaleja, PO Box 1100, 28800, Alcalá de Henares (Madrid), Spain aut NATIONALLICENCE 700 1- Jouve N. Department of Cell Biology and Genetics, University of Alcalá de Henares, 28871, Alcalá de Henares (Madrid), Spain aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/7(1996-11-01), 491-499 0967-3849 4:7<491 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer