caa a22 4500 477089631 CHVBK 20180405111512.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF02254965 doi (NATIONALLICENCE)springer-10.1007/BF02254965 Evidence for a megareplicon covering megabases of centromeric chromosome segments [Elektronische Daten] [Gy. Holló, J. Keresõ, T. Praznovszky, I. Cserpán, K. Fodor, R. Katona, E. Csonka, K. Fátyol, A. Szeles, A. Szalay, Gy. Hadlaczky] We have analysed the replication of the heterochromatic megachromosome that was formedde novo by a large-scale amplification process initiated in the centromeric region of mouse chromosome 7. The megachromosome is organized into amplicons ∼30 Mb in size, and each amplicon consists of two large inverted repeats delimited by a primary replication initiation site. Our results suggest that these segments represent a higher order replication unit (megareplicon) of the centromeric region of mouse chromosomes. Analysis of the replication of the megareplicons indicates that the pericentric heterochromatin and the centromere of mouse chromosomes begin to replicate early, and that their replication continues through approximately three-quarters of the S-phase. We suggest that a replication-directed mechanism may account for the initiation of large-scale amplification in the centromeric regions of mouse chromosomes, and may also explain the formation of new, stable chromosome segments and chromosomes. Rapid Science Publishers, 1996 amplification nationallicence centromere nationallicence heterochromatin nationallicence megareplicon nationallicence replication nationallicence Holló Gy Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut Keresõ J. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut Praznovszky T. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut Cserpán I. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut Fodor K. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut Katona R. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut Csonka E. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut Fátyol K. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut Szeles A. Microbiology and Tumorbiology Center (MTC), Karolinska Institutet, S-171 77, Stockholm, Sweden aut Szalay A. Center for Molecular Biology and Gene Therapy, Loma Linda University, School of Medicine, 92350, Loma Linda, CA, USA aut Hadlaczky Gy Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut Chromosome Research Kluwer Academic Publishers 4/3(1996-04-01), 240-247 0967-3849 4:3<240 1996 4 10577 https://doi.org/10.1007/BF02254965 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02254965 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Holló Gy Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Keresõ J. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Praznovszky T. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Cserpán I. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Fodor K. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Katona R. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Csonka E. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Fátyol K. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut NATIONALLICENCE 700 1- Szeles A. Microbiology and Tumorbiology Center (MTC), Karolinska Institutet, S-171 77, Stockholm, Sweden aut NATIONALLICENCE 700 1- Szalay A. Center for Molecular Biology and Gene Therapy, Loma Linda University, School of Medicine, 92350, Loma Linda, CA, USA aut NATIONALLICENCE 700 1- Hadlaczky Gy Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-6701, Szeged, Hungary aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/3(1996-04-01), 240-247 0967-3849 4:3<240 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer