caa a22 4500 477089674 CHVBK 20180405111513.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF02254959 doi (NATIONALLICENCE)springer-10.1007/BF02254959 Painting rye B chromosomes in wheat: interphase chromatin organization, nuclear disposition and association in plants with two, three or four Bs [Elektronische Daten] [L. Morais-Cecílio, M. Delgado, R. Jones, W. Viegas] The B chromosomes (Bs) of rye (Secale cereale) have been studied at interphase in terms of their chromatin organization, patterns of nuclear disposition and physical association in plants with two, three, and four Bs. The study was made in the Lindström strain of hexaploid wheat, which carries the rye Bs as an addition line, byin situ hybridization with a B-specific probe and by genomicin situ hybridization (GISH) with rye genomic DNA, enabling whole chromosome painting. Repetive sequences common to the As and Bs of rye allow for visualization of the rye B at interphase in the wheat background. A B-specific probe enables the orientation of two or more Bs to be determined, and the combination of both probes used together gives information on the disposition of the Bs and on their patterns of physical association within the nucleus. The Bs form linear ‘strings', and the ends of their long arms, which can be detected by the B-specific probe, are usually located within the hemisphere of the nucleus that has the least condensed chromatin. There is dose-dependent association, and even numbers (2B, 4B) have a greater preference for association than odd ones (3B). Rapid Science Publishers, 1996 B chromosomes nationallicence genomic in situ hybridization nationallicence nuclear architecture nationallicence Secale cereale nationallicence Triticum aestivum cv nationallicence Lindström nationallicence Morais-Cecílio L. Departmento de Botânica eEngenharia Biológia, Instituto Superior de Agronomia, Tapada da Ajuda, P-1399, Lisbon Codex, Portugal aut Delgado M. Departmento de Botânica eEngenharia Biológia, Instituto Superior de Agronomia, Tapada da Ajuda, P-1399, Lisbon Codex, Portugal aut Jones R. The Institute of Biological Sciences, University of Wales Aberystwyth, Sir George Stapledon Building, SY23 3DD, Aberystwyth, Wales, UK aut Viegas W. Departmento de Botânica eEngenharia Biológia, Instituto Superior de Agronomia, Tapada da Ajuda, P-1399, Lisbon Codex, Portugal aut Chromosome Research Kluwer Academic Publishers 4/3(1996-04-01), 195-200 0967-3849 4:3<195 1996 4 10577 https://doi.org/10.1007/BF02254959 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02254959 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morais-Cecílio L. Departmento de Botânica eEngenharia Biológia, Instituto Superior de Agronomia, Tapada da Ajuda, P-1399, Lisbon Codex, Portugal aut NATIONALLICENCE 700 1- Delgado M. Departmento de Botânica eEngenharia Biológia, Instituto Superior de Agronomia, Tapada da Ajuda, P-1399, Lisbon Codex, Portugal aut NATIONALLICENCE 700 1- Jones R. The Institute of Biological Sciences, University of Wales Aberystwyth, Sir George Stapledon Building, SY23 3DD, Aberystwyth, Wales, UK aut NATIONALLICENCE 700 1- Viegas W. Departmento de Botânica eEngenharia Biológia, Instituto Superior de Agronomia, Tapada da Ajuda, P-1399, Lisbon Codex, Portugal aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/3(1996-04-01), 195-200 0967-3849 4:3<195 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer