caa a22 4500 477089682 CHVBK 20180405111513.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF02254955 doi (NATIONALLICENCE)springer-10.1007/BF02254955 Heterochromatin and ribosomal genes in Asellus aquaticus (Crust. Isop.) [Elektronische Daten] [R. Barzotti, F. Pelliccia, A. Rocchi] In the present investigation chromosomal preparations ofAsellus aquaticus were sequentially stained with chromomycin A3 to reveal the heterochromatic areas, hybridizedin situ with rDNA probes in order to map the ribosomal genes and finally silver stained to check the transcriptional activity of these genes. The results indicate the existence of a substantial correspondence of location and size among the heterochromatic regions and the regions over which thein situ hybridization signals spread. The ribosomal genes, quite independently of their location in the secondary constriction, can be silver stained and thus appear to be transcriptionally active. The ribosomal sequences also hybridize to the entire heterochromatic areas observed on the probable Y chromosome identified in some males of a natural population. These rRNA genes are only rarely transcriptionally active. Rapid Science Publishers, 1996 Asellus nationallicence crustacean nationallicence heterochromatin nationallicence ribosomal genes nationallicence silver staining nationallicence Barzotti R. Dipartimento di Genetica e Biologia Molecolare e Centro di Genetica Evoluzionistica del CNR, Università ‘La Sapienza', 5 Ple A, Moro, 00185, Rome, Italy aut Pelliccia F. Dipartimento di Genetica e Biologia Molecolare e Centro di Genetica Evoluzionistica del CNR, Università ‘La Sapienza', 5 Ple A, Moro, 00185, Rome, Italy aut Rocchi A. Dipartimento di Genetica e Biologia Molecolare e Centro di Genetica Evoluzionistica del CNR, Università ‘La Sapienza', 5 Ple A, Moro, 00185, Rome, Italy aut Chromosome Research Kluwer Academic Publishers 4/3(1996-04-01), 177-181 0967-3849 4:3<177 1996 4 10577 https://doi.org/10.1007/BF02254955 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02254955 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Barzotti R. Dipartimento di Genetica e Biologia Molecolare e Centro di Genetica Evoluzionistica del CNR, Università ‘La Sapienza', 5 Ple A, Moro, 00185, Rome, Italy aut NATIONALLICENCE 700 1- Pelliccia F. Dipartimento di Genetica e Biologia Molecolare e Centro di Genetica Evoluzionistica del CNR, Università ‘La Sapienza', 5 Ple A, Moro, 00185, Rome, Italy aut NATIONALLICENCE 700 1- Rocchi A. Dipartimento di Genetica e Biologia Molecolare e Centro di Genetica Evoluzionistica del CNR, Università ‘La Sapienza', 5 Ple A, Moro, 00185, Rome, Italy aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/3(1996-04-01), 177-181 0967-3849 4:3<177 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer