caa a22 4500 477089712 CHVBK 20180405111513.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF02254964 doi (NATIONALLICENCE)springer-10.1007/BF02254964 De novo chromosome formations by large-scale amplification of the centromeric region of mouse chromosomes [Elektronische Daten] [J. Keresõ, T. Praznovszky, I. Cserpán, K. Fodor, R. Katona, E. Csonka, K. Fátyol, Gy. Holló, A. Szeles, A. Ross, A. Sumner, A. Szalay, Gy. Hadlaczky] Chromosomes formedde novo which originated from the centromeric region of mouse chromosome 7, have been analysed. These new chromosomes were formed by apparently similar large-scale amplification processes, and are organized into amplicons of ∼30 Mb. Centromeric satellite DNA was found to be the constant component of all amplicons. Satellite DNA sequences either bordered the large euchromatic amplicons (E-type amplification), or made up the bulk of the constitutive heterochromatic amplicons (H-type amplification). Detailed analysis of a heterochromatic megachromosome formedde novo by an H-type amplification revealed that it is composed of a tandem array of 10-12 large (∼30 Mb) amplicons each marked with integrated ‘foreign' DNA sequences at both ends. Each amplicon is a giant palindrome, consisting of two inverted doublets of ∼7.5-Mb blocks of satellite DNA. Our results indicate that the building units of the pericentric heterochromatin of mouse chromosomes are ∼7.5-Mb blocks of satellite DNA flanked by nonsatellite sequences. We suggest that the formationde novo of various chromosome segments and chromosomes seen in different cell lines may be the result of large-scale E- and H-type amplification initiated in the pericentric region of chromosomes. Rapid Science Publishers, 1996 amplification nationallicence centromere nationallicence chromosome formation nationallicence heterochromatin nationallicence satellite DNA nationallicence Keresõ J. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut Praznovszky T. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut Cserpán I. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut Fodor K. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut Katona R. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut Csonka E. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut Fátyol K. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut Holló Gy Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut Szeles A. Microbiology and Tumorbiology Center (MTC), Karolinska Institutet, S-171 77, Stockholm, Sweden aut Ross A. MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut Sumner A. MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut Szalay A. Center for Molecular Biology and Gene Therapy, Loma Linda University, School of Medicine, 92350, Loma Linda, CA, USA aut Hadlaczky Gy Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut Chromosome Research Kluwer Academic Publishers 4/3(1996-04-01), 226-239 0967-3849 4:3<226 1996 4 10577 https://doi.org/10.1007/BF02254964 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02254964 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Keresõ J. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut NATIONALLICENCE 700 1- Praznovszky T. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut NATIONALLICENCE 700 1- Cserpán I. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut NATIONALLICENCE 700 1- Fodor K. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut NATIONALLICENCE 700 1- Katona R. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut NATIONALLICENCE 700 1- Csonka E. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut NATIONALLICENCE 700 1- Fátyol K. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut NATIONALLICENCE 700 1- Holló Gy Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut NATIONALLICENCE 700 1- Szeles A. Microbiology and Tumorbiology Center (MTC), Karolinska Institutet, S-171 77, Stockholm, Sweden aut NATIONALLICENCE 700 1- Ross A. MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut NATIONALLICENCE 700 1- Sumner A. MRC Human Genetics Unit, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK aut NATIONALLICENCE 700 1- Szalay A. Center for Molecular Biology and Gene Therapy, Loma Linda University, School of Medicine, 92350, Loma Linda, CA, USA aut NATIONALLICENCE 700 1- Hadlaczky Gy Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, PO Box 521, H-67 01, Szeged, Hungary aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/3(1996-04-01), 226-239 0967-3849 4:3<226 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer