caa a22 4500 477089720 CHVBK 20180405111513.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF02254963 doi (NATIONALLICENCE)springer-10.1007/BF02254963 Zoo-FISH delineates conserved chromosomal segments in horse and man [Elektronische Daten] [Terje Raudsepp, Lutz Frönicke, Harry Scherthan, Ingemar Gustavsson, Bhanu Chowdhary] Human chromosome specific libraries (CSLs) were individually applied to equine metaphase chromosomes using the fluorescencein situ hybridization (FISH) technique. All CSLs, except Y, showed painting signals on one or several horse chromosomes. In total 43 conserved chromosoma segments were painted. Homoeology could not, however, be detected for some segments of the equine genome. This is most likely related to the very weak signals displayed by some libraries, rather than to the absence of similarity with the human genome. In spite of divergence from the human genome, dated 70-80 million years ago, a fairly high degree of synteny conservation was observed. In seven cases, whole chromosome synteny was detected between the two species. The comparative painting results agreed completely with the limited gene mapping data available in horses, and also enabled us provisionally to assign one linkage group (U2) and one syntenic group (NP, MPI, IDH2) to specific equine chromosomes. Chromosomal assignments of three other syntenic groups are also proposed. The findings of this study will be of significant use in the expansion of the hitherto poorly developed equine gene map. Rapid Science Publishers, 1996 chromosome painting nationallicence comparative mapping nationallicence horse nationallicence human nationallicence zoo-FISH nationallicence Raudsepp Terje Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, S-750 07, Uppsala, Sweden aut Frönicke Lutz Division of Human Biology and Human Genetics, University of Kaiserslautern, Erwin-Schroedingerstrasse, D-67663, Kaiserslautern, Germany aut Scherthan Harry Division of Human Biology and Human Genetics, University of Kaiserslautern, Erwin-Schroedingerstrasse, D-67663, Kaiserslautern, Germany aut Gustavsson Ingemar Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, S-750 07, Uppsala, Sweden aut Chowdhary Bhanu Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, S-750 07, Uppsala, Sweden aut Chromosome Research Kluwer Academic Publishers 4/3(1996-04-01), 218-225 0967-3849 4:3<218 1996 4 10577 https://doi.org/10.1007/BF02254963 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02254963 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Raudsepp Terje Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, S-750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Frönicke Lutz Division of Human Biology and Human Genetics, University of Kaiserslautern, Erwin-Schroedingerstrasse, D-67663, Kaiserslautern, Germany aut NATIONALLICENCE 700 1- Scherthan Harry Division of Human Biology and Human Genetics, University of Kaiserslautern, Erwin-Schroedingerstrasse, D-67663, Kaiserslautern, Germany aut NATIONALLICENCE 700 1- Gustavsson Ingemar Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, S-750 07, Uppsala, Sweden aut NATIONALLICENCE 700 1- Chowdhary Bhanu Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, S-750 07, Uppsala, Sweden aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/3(1996-04-01), 218-225 0967-3849 4:3<218 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer