caa a22 4500 477089755 CHVBK 20180405111513.0 cr unu---uuuuu 170330e19960401xx s 000 0 eng 10.1007/BF02254960 doi (NATIONALLICENCE)springer-10.1007/BF02254960 High-resolution fluorescence in situ hybridization of RBM - and TSPY -related cosmids on released Y chromatin in humans and pygmy chimpanzees [Elektronische Daten] [Christian Conrad, Thomas Hierl, Birgitta Gläser, Kay Taylor, Sylvia Zeitler, Ann Chandley, Werner Schempp] Applying two-colour fluorescencein situ hybridization (FISH) we simultaneously hybridizedRBM- andTSPY-related cosmids to Y chromosomes in prophase and to released Y chromatin in interphase nuclei of man and pygmy chimpanzee. Whereas, even on prophasic Y chromosomes, no resolution of the overlappingRBM andTSPY signal clusters could be achieved, theRBM andTSPY signals are completely separated from each other in our maximum released Y chromatin stretches in interphase nuclei. These results unequivocally lend support to the view that theRBM andTSPY families have an interspersed organization on the Y chromosomes of man and higher apes. Thus, the distribution ofRBM andTSPY signals might well go back to a common organization of these genes next to each other on an ancient Y chromosome. Rapid Science Publishers, 1996 high-resolution nationallicence fluorescence nationallicence in situ hybridization nationallicence spermatogenesis genes nationallicence Y chromosome evolution nationallicence Conrad Christian Institute of Human Genetics and Anthropology, University of Freiberg, Breisacher Straße 33, D-79106, Freiburg, Germany aut Hierl Thomas Institute of Human Genetics and Anthropology, University of Freiberg, Breisacher Straße 33, D-79106, Freiburg, Germany aut Gläser Birgitta Institute of Human Genetics and Anthropology, University of Freiberg, Breisacher Straße 33, D-79106, Freiburg, Germany aut Taylor Kay Galton Laboratory, University College London, Wolfson House, 4 Stephenson Way, NW1 2HE, London, UK aut Zeitler Sylvia Institute of Human Genetics and Anthropology, University of Freiberg, Breisacher Straße 33, D-79106, Freiburg, Germany aut Chandley Ann Galton Laboratory, University College London, Wolfson House, 4 Stephenson Way, NW1 2HE, London, UK aut Schempp Werner Institute of Human Genetics and Anthropology, University of Freiberg, Breisacher Straße 33, D-79106, Freiburg, Germany aut Chromosome Research Kluwer Academic Publishers 4/3(1996-04-01), 201-206 0967-3849 4:3<201 1996 4 10577 https://doi.org/10.1007/BF02254960 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02254960 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Conrad Christian Institute of Human Genetics and Anthropology, University of Freiberg, Breisacher Straße 33, D-79106, Freiburg, Germany aut NATIONALLICENCE 700 1- Hierl Thomas Institute of Human Genetics and Anthropology, University of Freiberg, Breisacher Straße 33, D-79106, Freiburg, Germany aut NATIONALLICENCE 700 1- Gläser Birgitta Institute of Human Genetics and Anthropology, University of Freiberg, Breisacher Straße 33, D-79106, Freiburg, Germany aut NATIONALLICENCE 700 1- Taylor Kay Galton Laboratory, University College London, Wolfson House, 4 Stephenson Way, NW1 2HE, London, UK aut NATIONALLICENCE 700 1- Zeitler Sylvia Institute of Human Genetics and Anthropology, University of Freiberg, Breisacher Straße 33, D-79106, Freiburg, Germany aut NATIONALLICENCE 700 1- Chandley Ann Galton Laboratory, University College London, Wolfson House, 4 Stephenson Way, NW1 2HE, London, UK aut NATIONALLICENCE 700 1- Schempp Werner Institute of Human Genetics and Anthropology, University of Freiberg, Breisacher Straße 33, D-79106, Freiburg, Germany aut NATIONALLICENCE 773 0- Chromosome Research Kluwer Academic Publishers 4/3(1996-04-01), 201-206 0967-3849 4:3<201 1996 4 10577 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer